North M~Vulpe CD, 2016

Pubmed ID 27909446
Title Functional Toxicogenomic Profiling Expands Insight into Modulators of Formaldehyde Toxicity in Yeast.
Authors Matthew North, Brandon D Gaytán, Carlos Romero, Vanessa Y De La Rosa, Alex Loguinov, Martyn T Smith, Luoping Zhang, Chris D Vulpe
Abstract Saccharomyces cerevisiae to identify genes and cellular processes modulating the cellular toxicity of FA. Our results demonstrate mutant strains deficient in multiple DNA repair pathways-including homologous recombination, single strand annealing, and postreplication repair-were sensitive to FA, indicating FA may cause various forms of DNA damage in yeast. The SKI complex and its associated factors, which regulate mRNA degradation by the exosome, were also required for FA tolerance, suggesting FA may have unappreciated effects on RNA stability. Furthermore, various strains involved in osmoregulation and stress response were sensitive to FA. Together, our results are generally consistent with FA-mediated damage to both DNA and RNA. Considering DNA repair and RNA degradation pathways are evolutionarily conserved from yeast to humans, mechanisms of FA toxicity identified in yeast may be relevant to human disease and genetic susceptibility.
Citation Front Genet 2016; 7:200

Datasets

Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
North M~Vulpe CD, 2016 growth (relative abundance in pooled culture) formaldehyde [0.6 mM], time [5 gen] YPD hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2016 growth (relative abundance in pooled culture) formaldehyde [0.3mM], time [5 gen] YPD hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2016 growth (relative abundance in pooled culture) formaldehyde [0.15mM], time [5 gen] YPD hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2016 growth (relative abundance in pooled culture) formaldehyde [0.6 mM], time [15 gen] YPD hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2016 growth (relative abundance in pooled culture) formaldehyde [0.3 mM], time [15 gen] YPD hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2016 growth (relative abundance in pooled culture) formaldehyde [0.15 mM], time [15 gen] YPD hom ~4,607 Quantitative only for hits

Curation history

Data

Jan. 13, 2017 Waiting for tested.
April 25, 2017 Request abandoned.
April 25, 2017 Ready to load.
May 9, 2017 Loaded.

Tested strains

Jan. 13, 2017 To request.
April 25, 2017 Request abandoned.