Irwin B~Sandmeyer S, 2005

Pubmed ID 15837808
Title Retroviruses and yeast retrotransposons use overlapping sets of host genes.
Authors Becky Irwin, Michael Aye, Pierre Baldi, Nadejda Beliakova-Bethell, Henry Cheng, Yimeng Dou, Willy Liou, Suzanne Sandmeyer
Abstract A collection of 4457 Saccharomyces cerevisiae mutants deleted for nonessential genes was screened for mutants with increased or decreased mobilization of the gypsylike retroelement Ty3. Of these, 64 exhibited increased and 66 decreased Ty3 transposition compared with the parental strain. Genes identified in this screen were grouped according to function by using GOnet software developed as part of this study. Gene clusters were related to chromatin and transcript elongation, translation and cytoplasmic RNA processing, vesicular trafficking, nuclear transport, and DNA maintenance. Sixty-six of the mutants were tested for Ty3 proteins and cDNA. Ty3 cDNA and transposition were increased in mutants affected in nuclear pore biogenesis and in a subset of mutants lacking proteins that interact physically or genetically with a replication clamp loader. Our results suggest that nuclear entry is linked mechanistically to Ty3 cDNA synthesis but that host replication factors antagonize Ty3 replication. Some of the factors we identified have been previously shown to affect Ty1 transposition and others to affect retroviral budding. Host factors, such as these, shared by distantly related Ty retroelements and retroviruses are novel candidates for antiviral targets.
Citation Genome Res. 2005; 15:641-54

Datasets

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Papers Phenotype Conditions Collection Tested mutants Data Details
Irwin B~Sandmeyer S, 2005 transposable element transposition (Ty3) standard [standard] hap a ~4,457 Discrete

Curation history

March 5, 2014 Tested strains requested.
March 5, 2014 Data waiting for tested.
Nov. 11, 2014 Tested strains requested.
Jan. 28, 2015 Tested strains abandoned.
Jan. 28, 2015 Data to load.
Jan. 29, 2017 Data loaded.