Vlaming H~Leeuwen F, 2016

Pubmed ID 27922451
Title Direct screening for chromatin status on DNA barcodes in yeast delineates the regulome of H3K79 methylation by Dot1.
Authors Hanneke Vlaming, Thom M Molenaar, Tibor van Welsem, Deepani W Poramba-Liyanage, Desiree E Smith, Arno Velds, Liesbeth Hoekman, Tessy Korthout, Sjoerd Hendriks, A F Maarten Altelaar, Fred van Leeuwen
Abstract Given the frequent misregulation of chromatin in cancer, it is important to understand the cellular mechanisms that regulate chromatin structure. However, systematic screening for epigenetic regulators is challenging and often relies on laborious assays or indirect reporter read-outs. Here we describe a strategy, Epi-ID, to directly assess chromatin status in thousands of mutants. In Epi-ID, chromatin status on DNA barcodes is interrogated by chromatin immunoprecipitation followed by deep sequencing, allowing for quantitative comparison of many mutants in parallel. Screening of a barcoded yeast knock-out collection for regulators of histone H3K79 methylation by Dot1 identified all known regulators as well as novel players and processes. These include histone deposition, homologous recombination, and adenosine kinase, which influences the methionine cycle. Gcn5, the acetyltransferase within the SAGA complex, was found to regulate histone methylation and H2B ubiquitination. The concept of Epi-ID is widely applicable and can be readily applied to other chromatin features.
Citation Elife 2016; 5:.
Notes we applied Epi-ID to delineate the regulome of H3K79 methylation in budding yeast


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Papers Phenotype Conditions Collection Tested mutants Data Details
Vlaming H~Leeuwen F, 2016 protein/peptide methylation (H3K79) standard [standard] hap alpha 4,206 Quantitative

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Jan. 13, 2017 Tested strains clarification needed.
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June 1, 2017 Tested strains loaded.
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