Mulleder M~Ralser M, 2016

Pubmed ID 27693354
Title Functional Metabolomics Describes the Yeast Biosynthetic Regulome.
Authors Michael Mülleder, Enrica Calvani, Mohammad Tauqeer Alam, Richard Kangda Wang, Florian Eckerstorfer, Aleksej Zelezniak, Markus Ralser
Abstract Genome-metabolism interactions enable cell growth. To probe the extent of these interactions and delineate their functional contributions, we quantified the Saccharomyces amino acid metabolome and its response to systematic gene deletion. Over one-third of coding genes, in particular those important for chromatin dynamics, translation, and transport, contribute to biosynthetic metabolism. Specific amino acid signatures characterize genes of similar function. This enabled us to exploit functional metabolomics to connect metabolic regulators to their effectors, as exemplified by TORC1, whose inhibition in exponentially growing cells is shown to match an interruption in endomembrane transport. Providing orthogonal information compared to physical and genetic interaction networks, metabolomic signatures cluster more than half of the so far uncharacterized yeast genes and provide functional annotation for them. A major part of coding genes is therefore participating in gene-metabolism interactions that expose the metabolism regulatory network and enable access to an underexplored space in gene function.
Citation Cell 2016; 167:553-565.e12

Datasets

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Paper Phenotype Condition Reference Collection Tested mutants Data Details
Mulleder M~Ralser M, 2016 abundance of alanine SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of aspartic acid SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of glutamate SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of phenylalanine SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of glycine SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of histidine SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of isoleucine SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of lysine SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of leucine SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of methionine SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of asparagine SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of proline SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of glutamine SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of arginine SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of serine SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of threonine SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of valine SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of tryptophan SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of tyrosine SD hap a 4,678 Quantitative

Curation history

Tested strains

May 19, 2017 Ready to load.
May 26, 2017 Loaded.

Data

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May 26, 2017 Loaded.