Mulleder M~Ralser M, 2016

Pubmed ID 27693354
Title Functional Metabolomics Describes the Yeast Biosynthetic Regulome.
Authors Michael Mülleder, Enrica Calvani, Mohammad Tauqeer Alam, Richard Kangda Wang, Florian Eckerstorfer, Aleksej Zelezniak, Markus Ralser
Abstract Genome-metabolism interactions enable cell growth. To probe the extent of these interactions and delineate their functional contributions, we quantified the Saccharomyces amino acid metabolome and its response to systematic gene deletion. Over one-third of coding genes, in particular those important for chromatin dynamics, translation, and transport, contribute to biosynthetic metabolism. Specific amino acid signatures characterize genes of similar function. This enabled us to exploit functional metabolomics to connect metabolic regulators to their effectors, as exemplified by TORC1, whose inhibition in exponentially growing cells is shown to match an interruption in endomembrane transport. Providing orthogonal information compared to physical and genetic interaction networks, metabolomic signatures cluster more than half of the so far uncharacterized yeast genes and provide functional annotation for them. A major part of coding genes is therefore participating in gene-metabolism interactions that expose the metabolism regulatory network and enable access to an underexplored space in gene function.
Citation Cell 2016; 167:553-565.e12

Datasets

Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Mulleder M~Ralser M, 2016 abundance of alanine standard SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of aspartic acid standard SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of glutamate standard SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of phenylalanine standard SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of glycine standard SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of histidine standard SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of isoleucine standard SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of lysine standard SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of leucine standard SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of methionine standard SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of asparagine standard SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of proline standard SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of glutamine standard SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of arginine standard SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of serine standard SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of threonine standard SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of valine standard SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of tryptophan standard SD hap a 4,678 Quantitative
Mulleder M~Ralser M, 2016 abundance of tyrosine standard SD hap a 4,678 Quantitative

Curation history

Data

May 19, 2017 Ready to load.
May 26, 2017 Loaded.

Tested strains

May 19, 2017 Ready to load.
May 26, 2017 Loaded.