Yofe I~Thoms S, 2017

Pubmed ID 28049721
Title Pex35 is a regulator of peroxisome abundance.
Authors Ido Yofe, Kareem Soliman, Silvia G Chuartzman, Bruce Morgan, Uri Weill, Eden Yifrach, Tobias P Dick, Sara J Cooper, Christer S Ejsing, Maya Schuldiner, Einat Zalckvar, Sven Thoms
Abstract Peroxisomes are cellular organelles with vital functions in lipid, amino acid and redox metabolism. The cellular formation and dynamics of peroxisomes are governed by PEX genes; however, the regulation of peroxisome abundance is still poorly understood. Here, we use a high-content microscopy screen in Saccharomyces cerevisiae to identify new regulators of peroxisome size and abundance. Our screen led to the identification of a previously uncharacterized gene, which we term PEX35, which affects peroxisome abundance. PEX35 encodes a peroxisomal membrane protein, a remote homolog to several curvature-generating human proteins. We systematically characterized the genetic and physical interactome as well as the metabolome of mutants in PEX35, and we found that Pex35 functionally interacts with the vesicle-budding-inducer Arf1. Our results highlight the functional interaction between peroxisomes and the secretory pathway.
Citation J. Cell. Sci. 2017; 130:791-804

Datasets

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Papers Phenotype Conditions Collection Tested mutants Data Details
Yofe I~Thoms S, 2017 number of peroxisomes standard [standard] hap a 4,223 Quantitative
Yofe I~Thoms S, 2017 size of peroxisomes standard [standard] hap a 4,223 Quantitative

Curation history

June 8, 2017 Tested strains to load.
June 8, 2017 Data to load.
June 13, 2017 Tested strains loaded.
June 13, 2017 Data loaded.