Marmiroli M~Marmiroli N, 2016

Pubmed ID 25938282
Title A genome-wide nanotoxicology screen of Saccharomyces cerevisiae mutants reveals the basis for cadmium sulphide quantum dot tolerance and sensitivity.
Authors M Marmiroli, L Pagano, F Pasquali, A Zappettini, V Tosato, C V Bruschi, N Marmiroli
Abstract The use of cadmium sulphide quantum dots (CdS QDs) is increasing, particularly in the electronics industry. Their size (1-10‚ÄČnm in diameter) is, however, such that they can be taken up by living cells. Here, a bakers' yeast (Saccharomyces cerevisiae) deletion mutant collection has been exploited to provide a high-throughput means of revealing the genetic basis for tolerance/susceptibility to CdS QD exposure. The deletion of 112 genes, some associated with the abiotic stress response, some with various metabolic processes, some with mitochondrial organization, some with transport and some with DNA repair, reduced the level of tolerance to CdS QDs. A gene ontology analysis highlighted the role of oxidative stress in determining the cellular response. The transformation of sensitive mutants with centromeric plasmids harbouring DNA from a wild type strain restored the wild type growth phenotype when the complemented genes encoded either HSC82, DSK2 or ALD3. The use of these simple eukaryote knock-out mutants for functional toxicogenomic analysis will inform studies focusing on higher organisms.
Citation Nanotoxicology 2016; 10:84-93

Datasets

Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Marmiroli M~Marmiroli N, 2016 growth (colony size) cadsium sulphide quantum dots [200 mg/L] hap alpha ~4,688 None

Curation history

Data

June 23, 2017 To request.
June 28, 2017 Request sent.
Aug. 28, 2017 Request sent.
Sept. 4, 2017 Not available.

Tested strains

June 23, 2017 To request.
June 28, 2017 Request sent.
Aug. 28, 2017 Request sent.
Sept. 4, 2017 Not available.