Sambade M~Kane PM, 2005

Pubmed ID 15937126
Title A genomic screen for yeast vacuolar membrane ATPase mutants.
Authors Maria Sambade, Mercedes Alba, Anne M Smardon, Robert W West, Patricia M Kane
Abstract V-ATPases acidify multiple organelles, and yeast mutants lacking V-ATPase activity exhibit a distinctive set of growth defects. To better understand the requirements for organelle acidification and the basis of these growth phenotypes, approximately 4700 yeast deletion mutants were screened for growth defects at pH 7.5 in 60 mm CaCl(2). In addition to 13 of 16 mutants lacking known V-ATPase subunits or assembly factors, 50 additional mutants were identified. Sixteen of these also grew poorly in nonfermentable carbon sources, like the known V-ATPase mutants, and were analyzed further. The cwh36Delta mutant exhibited the strongest phenotype; this mutation proved to disrupt a previously uncharacterized V-ATPase subunit. A small subset of the mutations implicated in vacuolar protein sorting, vps34Delta, vps15Delta, vps45Delta, and vps16Delta, caused both Vma- growth phenotypes and lower V-ATPase activity in isolated vacuoles, as did the shp1Delta mutation, implicated in both protein sorting and regulation of the Glc7p protein phosphatase. These proteins may regulate V-ATPase targeting and/or activity. Eight mutants showed a Vma- growth phenotype but no apparent defect in vacuolar acidification. Like V-ATPase-deficient mutants, most of these mutants rely on calcineurin for growth, particularly at high pH. A requirement for constitutive calcineurin activation may be the predominant physiological basis of the Vma- growth phenotype.
Citation Genetics 2005; 170:1539-51

Datasets

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Papers Phenotype Conditions Collection Tested mutants Data Details
Sambade M~Kane PM, 2005 growth (colony size) calcium chloride [60 mM], pH [7.5] hap a 4,787 Discrete

Curation history

March 7, 2014 Tested strains requested.
March 7, 2014 Data waiting for tested.
March 13, 2014 Tested strains to load.
March 13, 2014 Data to load.
April 1, 2014 Tested strains loaded.
April 1, 2014 Data loaded.