Eapen VV~Haber JE, 2017

Pubmed ID 28154131
Title A pathway of targeted autophagy is induced by DNA damage in budding yeast.
Authors Vinay V Eapen, David P Waterman, Amélie Bernard, Nathan Schiffmann, Enrich Sayas, Roarke Kamber, Brenda Lemos, Gonen Memisoglu, Jessie Ang, Allison Mazella, Silvia G Chuartzman, Robbie J Loewith, Maya Schuldiner, Vladimir Denic, Daniel J Klionsky, James E Haber
Abstract Autophagy plays a central role in the DNA damage response (DDR) by controlling the levels of various DNA repair and checkpoint proteins; however, how the DDR communicates with the autophagy pathway remains unknown. Using budding yeast, we demonstrate that global genotoxic damage or even a single unrepaired double-strand break (DSB) initiates a previously undescribed and selective pathway of autophagy that we term genotoxin-induced targeted autophagy (GTA). GTA requires the action primarily of Mec1/ATR and Rad53/CHEK2 checkpoint kinases, in part via transcriptional up-regulation of central autophagy proteins. GTA is distinct from starvation-induced autophagy. GTA requires Atg11, a central component of the selective autophagy machinery, but is different from previously described autophagy pathways. By screening a collection of ∼6,000 yeast mutants, we identified genes that control GTA but do not significantly affect rapamycin-induced autophagy. Overall, our findings establish a pathway of autophagy specific to the DNA damage response.
Citation Proc. Natl. Acad. Sci. U.S.A. 2017; 114:E1158-E1167

Datasets

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Papers Phenotype Conditions Collection Tested mutants Data Details
Eapen VV~Haber JE, 2017 protein localization to vacuole (GFP-Atg8) methylmethane sulphonate [0.04%] hap a N/A None

Curation history

Data

Aug. 31, 2017 Data to request.
Sept. 24, 2017 Data requested.
Jan. 30, 2018 Data requested.
April 3, 2018 Data abandoned.

Tested strains

Aug. 31, 2017 Tested strains to request.
Sept. 24, 2017 Tested strains requested.
Jan. 30, 2018 Tested strains requested.
April 3, 2018 Tested strains abandoned.