Cohen Y~Schuldiner M, 2014

Pubmed ID 24722918
Title Peroxisomes are juxtaposed to strategic sites on mitochondria.
Authors Yifat Cohen, Yoel Alexander Klug, Lazar Dimitrov, Zohar Erez, Silvia G Chuartzman, Dalia Elinger, Ido Yofe, Kareem Soliman, Jutta Gärtner, Sven Thoms, Randy Schekman, Yael Elbaz-Alon, Einat Zalckvar, Maya Schuldiner
Abstract Peroxisomes are ubiquitous and dynamic organelles that house many important pathways of cellular metabolism. In recent years it has been demonstrated that mitochondria are tightly connected with peroxisomes and are defective in several peroxisomal diseases. Indeed, these two organelles share metabolic routes as well as resident proteins and, at least in mammals, are connected via a vesicular transport pathway. However the exact extent of cross-talk between peroxisomes and mitochondria remains unclear. Here we used a combination of high throughput genetic manipulations of yeast libraries alongside high content screens to systematically unravel proteins that affect the transport of peroxisomal proteins and peroxisome biogenesis. Follow up work on the effector proteins that were identified revealed that peroxisomes are not randomly distributed in cells but are rather localized to specific mitochondrial subdomains such as mitochondria-ER junctions and sites of acetyl-CoA synthesis. Our approach highlights the intricate geography of the cell and suggests an additional layer of organization as a possible way to enable efficient metabolism. Our findings pave the way for further studying the machinery aligning mitochondria and peroxisomes, the role of the juxtaposition, as well as its regulation during various metabolic conditions. More broadly, the approaches used here can be easily applied to study any organelle of choice, facilitating the discovery of new aspects in cell biology.
Citation Mol Biosyst 2014; 10:1742-8

Datasets

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Cohen Y~Schuldiner M, 2014 aberrant peroxisomes (Cherry-PTS1 GFP-Ant1) standard hap a 4,885 Discrete
Cohen Y~Schuldiner M, 2014 number of peroxisomes (Cherry-PTS1 GFP-Ant1) standard hap a 4,885 Discrete
Cohen Y~Schuldiner M, 2014 transport to/from peroxisomes (Pts1) standard hap a 4,885 Discrete

Curation history

Data

Oct. 10, 2017 Waiting for tested.
Feb. 26, 2018 Ready to load.
Feb. 27, 2018 Loaded.

Tested strains

Oct. 10, 2017 To request.
Feb. 22, 2018 Request sent.
Feb. 26, 2018 Ready to load.
Feb. 27, 2018 Loaded.