Michaillat L~Mayer A, 2013

Pubmed ID 23383298
Title Identification of genes affecting vacuole membrane fragmentation in Saccharomyces cerevisiae.
Authors Lydie Michaillat, Andreas Mayer
Abstract The equilibrium of membrane fusion and fission influences the volume and copy number of organelles. Fusion of yeast vacuoles has been well characterized but their fission and the mechanisms determining vacuole size and abundance remain poorly understood. We therefore attempted to systematically characterize factors necessary for vacuole fission. Here, we present results of an in vivo screening for deficiencies in vacuolar fragmentation activity of an ordered collection deletion mutants, representing 4881 non-essential genes of the yeast Saccharomyces cerevisiae. The screen identified 133 mutants with strong defects in vacuole fragmentation. These comprise numerous known fragmentation factors, such as the Fab1p complex, Tor1p, Sit4p and the V-ATPase, thus validating the approach. The screen identified many novel factors promoting vacuole fragmentation. Among those are 22 open reading frames of unknown function and three conspicuous clusters of proteins with known function. The clusters concern the ESCRT machinery, adaptins, and lipases, which influence the production of diacylglycerol and phosphatidic acid. A common feature of these factors of known function is their capacity to change membrane curvature, suggesting that they might promote vacuole fragmentation via this property.
Citation PLoS ONE 2013; 8:e54160


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Papers Phenotype Conditions Collection Tested mutants Data Details
Michaillat L~Mayer A, 2013 vacuolar fragmentation (FM4-64 staining) sodium chloride [0.4 M] hap a ~4,881 Discrete

Curation history

Sept. 14, 2017 Tested strains to request.
Sept. 14, 2017 Data waiting for tested.
Jan. 19, 2018 Tested strains requested.