Prescott TA~Simmonds MS, 2014

Pubmed ID 24374339
Title A yeast chemical genetics approach identifies the compound 3,4,5-trimethoxybenzyl isothiocyanate as a calcineurin inhibitor.
Authors Thomas A K Prescott, Barry Panaretou, Nigel C Veitch, Monique S J Simmonds
Abstract The phosphatase enzyme calcineurin controls gene expression in a variety of biological contexts however few potent inhibitors are currently available. A screen of 360 plant extracts for inhibition of calcineurin-dependent gene expression in the model organism Saccharomyces cerevisiae identified the compound 3,4,5-trimethoxybenzyl isothiocyanate as an inhibitor. The compound was subsequently shown to inhibit human calcineurin via a mixed inhibition mechanism. To gain further mechanistic insight a yeast haploinsufficiency screen of 1152 deletion strains was carried out using a novel liquid medium screening method. The resulting haploinsufficiency profile is similar to that reported for the known calcineurin inhibitor FK506.
Citation FEBS Lett. 2014; 588:455-8

Datasets

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Papers Phenotype Conditions Collection Tested mutants Data Details
Prescott TA~Simmonds MS, 2014 growth (culture turbidity) 3,4,5-trimethoxybenzyl isothiocyanate [8.4-16.7 uM] het 1,124 Discrete

Curation history

Sept. 14, 2017 Tested strains to request.
Sept. 14, 2017 Data waiting for tested.
Jan. 15, 2018 Tested strains to load.
Jan. 15, 2018 Data to load.
Jan. 18, 2018 Tested strains loaded.
Jan. 18, 2018 Data loaded.