Vizeacoumar FJ~Andrews BJ, 2010

Pubmed ID 20065090
Title Integrating high-throughput genetic interaction mapping and high-content screening to explore yeast spindle morphogenesis.
Authors Franco J Vizeacoumar, Nydia van Dyk, Frederick S Vizeacoumar, Vincent Cheung, Jingjing Li, Yaroslav Sydorskyy, Nicolle Case, Zhijian Li, Alessandro Datti, Corey Nislow, Brian Raught, Zhaolei Zhang, Brendan Frey, Kerry Bloom, Charles Boone, Brenda J Andrews
Abstract We describe the application of a novel screening approach that combines automated yeast genetics, synthetic genetic array (SGA) analysis, and a high-content screening (HCS) system to examine mitotic spindle morphogenesis. We measured numerous spindle and cellular morphological parameters in thousands of single mutants and corresponding sensitized double mutants lacking genes known to be involved in spindle function. We focused on a subset of genes that appear to define a highly conserved mitotic spindle disassembly pathway, which is known to involve Ipl1p, the yeast aurora B kinase, as well as the cell cycle regulatory networks mitotic exit network (MEN) and fourteen early anaphase release (FEAR). We also dissected the function of the kinetochore protein Mcm21p, showing that sumoylation of Mcm21p regulates the enrichment of Ipl1p and other chromosomal passenger proteins to the spindle midzone to mediate spindle disassembly. Although we focused on spindle disassembly in a proof-of-principle study, our integrated HCS-SGA method can be applied to virtually any pathway, making it a powerful means for identifying specific cellular functions.
Citation J. Cell Biol. 2010; 188:69-81

Datasets

Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Vizeacoumar FJ~Andrews BJ, 2010 unbud cell mean area standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 unbud cell mean perimeter standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 unbud cell mean length standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 unbud cell mean breadth standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 unbud cell mean shapefactor standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 unbud cell mean ell. form factor standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 unbud cell mean equiv. prolate vol. standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 unbud cell mean oblate vol. standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud mean mother area standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud mean mother perimeter standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud mean mother length standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud mean mother breadth standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud mean mother shape factor standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud mean mother ell. form factor standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud mean mother equiv. prolate vol. standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud mean mother equiv. oblate vol. standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud mean daughter area standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud mean daughter perimeter standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud mean daughter length standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud mean daughter breadth standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud mean daughter shape factor standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud mean daughter ell. form factor standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud mean daughter equiv. prolate vol. standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud mean daughter equiv. oblate vol. standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud mean budneck width standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud mean mother area standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud mean mother perimeter standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud mean mother length standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud mean mother breadth standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud mean mother shape factor standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud mean mother ell. form factor standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud mean mother equiv. prolate vol. standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud mean mother equiv. oblate vol. standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud mean daughter area standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud mean daughter perimeter standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud mean daughter length standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud mean daughter breadth standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud mean daughter shape factor standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud mean daughter ell. form factor standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud mean daughter equiv. prolate vol. standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud mean daughter equiv. oblate vol. standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud mean budneck width standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud anaphase spindle mean fiber length standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud anaphase spindle mean spindle dist to budneck standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud anaphase spindle mean spindle orientation standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud metaphase spindle mean fiber length standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud metaphase spindle mean spindle dist to budneck standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 large bud metaphase spindle mean spindle orientation standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud anaphase spindle mean fiber length standard hap a 4,267 Quantitative
Vizeacoumar FJ~Andrews BJ, 2010 medium bud anaphase spindle mean spindle dist to budneck standard hap a 4,267 Quantitative
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Curation history

Data

Jan. 30, 2018 Loaded.

Tested strains

Jan. 30, 2018 Loaded.