Huang S~O'Shea EK, 2005

Pubmed ID 15695358
Title A systematic high-throughput screen of a yeast deletion collection for mutants defective in PHO5 regulation.
Authors Sidong Huang, Erin K O'Shea
Abstract In response to phosphate limitation, Saccharomyces cerevisiae induces transcription of a set of genes important for survival. One of these genes is PHO5, which encodes a secreted acid phosphatase. A phosphate-responsive signal transduction pathway (the PHO pathway) mediates this response through three central components: a cyclin-dependent kinase (CDK), Pho85; a cyclin, Pho80; and a CDK inhibitor (CKI), Pho81. While signaling downstream of the Pho81/Pho80/Pho85 complex to PHO5 expression has been well characterized, little is known about factors acting upstream of these components. To identify missing factors involved in the PHO pathway, we carried out a high-throughput, quantitative enzymatic screen of a yeast deletion collection, searching for novel mutants defective in expression of PHO5. As a result of this study, we have identified at least nine genes that were previously not known to regulate PHO5 expression. The functional diversity of these genes suggests that the PHO pathway is networked with other important cellular signaling pathways. Among these genes, ADK1 and ADO1, encoding an adenylate kinase and an adenosine kinase, respectively, negatively regulate PHO5 expression and appear to function upstream of PHO81.
Citation Genetics 2005; 169:1859-71
Data abstract Deletion collection strains were assayed for phosphatase activity.


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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Huang S~O'Shea EK, 2005 phosphatase activity of a specific protein (Pho5) standard SC (no phosphate) hap a 4,785 Quantitative

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