Bell G, 2010

Pubmed ID 20129976
Title Experimental genomics of fitness in yeast.
Authors Graham Bell
Abstract The set of single-gene deletions in yeast can be used to evaluate the effect of mutation on fitness over the whole genome. The measurement of growth in pure culture or relative growth in mixtures has confirmed that most deletions have little effect in laboratory culture. Moreover, there is a sharp distinction between lethality and a very mild impairment of growth, with very few intermediate cases. Different components of fitness, such as growth rate and yield, are positively correlated. Growth is also positively correlated across environments, although new conditions of growth usually identify a few conditionally impaired strains. Double mutants on average show alleviating epistasis, although a few per cent of combinations are synthetic lethal. The properties of the yeast deletion set provide us with the first genome-wide account of fitness, although transferring these conclusions to the field is a task for the future.
Citation Proc. Biol. Sci. 2010; 277:1459-67

Datasets

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Bell G, 2010 growth (culture turbidity) standard YPD hap a ~4,846 Quantitative
Bell G, 2010 growth (culture turbidity) standard SD hap a ~4,846 Quantitative
Bell G, 2010 growth (culture turbidity) nitrogen starvation [unknown] hap a ~4,846 None
Bell G, 2010 growth (culture turbidity) standard YPE [2%] hap a ~4,846 Quantitative
Bell G, 2010 growth (culture turbidity) temperature [37ÂșC] hap a ~4,846 Quantitative
Bell G, 2010 growth (culture turbidity) standard maple sap hap a ~4,846 Quantitative
Bell G, 2010 growth (culture turbidity) standard infusion of leaves hap a ~4,846 Quantitative
Bell G, 2010 growth (culture turbidity) standard infusion of bark hap a ~4,846 Quantitative
Bell G, 2010 growth (culture turbidity) standard birch sap hap a ~4,846 Quantitative

Curation history

Data

Feb. 23, 2018 To request.
March 5, 2018 Request sent.
Aug. 27, 2018 Request sent.

Tested strains

Feb. 23, 2018 To request.
March 5, 2018 Request sent.
Aug. 27, 2018 Request sent.