Salignon J~Yvert G, 2018

Pubmed ID 29507053
Title Genomics of cellular proliferation in periodic environmental fluctuations.
Authors Jérôme Salignon, Magali Richard, Etienne Fulcrand, Hélène Duplus-Bottin, Gaël Yvert
Abstract Living systems control cell growth dynamically by processing information from their environment. Although responses to a single environmental change have been intensively studied, little is known about how cells react to fluctuating conditions. Here, we address this question at the genomic scale by measuring the relative proliferation rate (fitness) of 3,568 yeast gene deletion mutants in out-of-equilibrium conditions: periodic oscillations between two environmental conditions. In periodic salt stress, fitness and its genetic variance largely depended on the oscillating period. Surprisingly, dozens of mutants displayed pronounced hyperproliferation under short stress periods, revealing unexpected controllers of growth under fast dynamics. We validated the implication of the high-affinity cAMP phosphodiesterase and of a regulator of protein translocation to mitochondria in this group. Periodic oscillations of extracellular methionine, a factor unrelated to salinity, also altered fitness but to a lesser extent and for different genes. The results illustrate how natural selection acts on mutations in a dynamic environment, highlighting unsuspected genetic vulnerabilities to periodic stress in molecular processes that are conserved across all eukaryotes.
Citation Mol. Syst. Biol. 2018; 14:e7823

Datasets

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Salignon J~Yvert G, 2018 growth (relative abundance in pooled culture) standard SC hom 3,540 Quantitative
Salignon J~Yvert G, 2018 growth (relative abundance in pooled culture) sodium chloride [0.2 M] SC hom 3,540 Quantitative
Salignon J~Yvert G, 2018 growth (relative abundance in pooled culture) salt stress oscillation SC hom 3,540 Quantitative
Salignon J~Yvert G, 2018 growth (relative abundance in pooled culture) standard SC - Met hom 3,540 Quantitative
Salignon J~Yvert G, 2018 growth (relative abundance in pooled culture) methionine [1 mM] SC - Met hom 3,540 Quantitative
Salignon J~Yvert G, 2018 growth (relative abundance in pooled culture) methionine oscillation SC - Met hom 3,540 Quantitative
Salignon J~Yvert G, 2018 growth (relative abundance in pooled culture) salt stress oscillation SC hom 3,540 Quantitative
Salignon J~Yvert G, 2018 growth (relative abundance in pooled culture) salt stress oscillation SC hom 3,540 Quantitative
Salignon J~Yvert G, 2018 growth (relative abundance in pooled culture) salt stress oscillation SC hom 3,540 Quantitative
Salignon J~Yvert G, 2018 growth (relative abundance in pooled culture) salt stress oscillation SC hom 3,540 Quantitative
Salignon J~Yvert G, 2018 growth (relative abundance in pooled culture) methionine oscillation SC - Met hom 3,540 Quantitative
Salignon J~Yvert G, 2018 growth (relative abundance in pooled culture) methionine oscillation SC - Met hom 3,540 Quantitative
Salignon J~Yvert G, 2018 growth (relative abundance in pooled culture) methionine oscillation SC - Met hom 3,540 Quantitative
Salignon J~Yvert G, 2018 growth (relative abundance in pooled culture) methionine oscillation SC - Met hom 3,540 Quantitative

Curation history

Data

March 9, 2018 Ready to load.
April 4, 2018 Loaded.

Tested strains

March 9, 2018 Ready to load.
April 4, 2018 Loaded.