Villa-Garcia MJ~Henry SA, 2011

Pubmed ID 21136082
Title Genome-wide screen for inositol auxotrophy in Saccharomyces cerevisiae implicates lipid metabolism in stress response signaling.
Authors Manuel J Villa-García, Myung Sun Choi, Flora I Hinz, María L Gaspar, Stephen A Jesch, Susan A Henry
Abstract Inositol auxotrophy (Ino(-) phenotype) in budding yeast has classically been associated with misregulation of INO1 and other genes involved in lipid metabolism. To identify all non-essential yeast genes that are necessary for growth in the absence of inositol, we carried out a genome-wide phenotypic screening for deletion mutants exhibiting Ino(-) phenotypes under one or more growth conditions. We report the identification of 419 genes, including 385 genes not previously reported, which exhibit this phenotype when deleted. The identified genes are involved in a wide range of cellular processes, but are particularly enriched in those affecting transcription, protein modification, membrane trafficking, diverse stress responses, and lipid metabolism. Among the Ino(-) mutants involved in stress response, many exhibited phenotypes that are strengthened at elevated temperature and/or when choline is present in the medium. The role of inositol in regulation of lipid metabolism and stress response signaling is discussed.
Citation Mol. Genet. Genomics 2011; 285:125-49

Datasets

Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Villa-Garcia MJ~Henry SA, 2011 growth (spot assay) temperature [30°C] SC - Ino - Cho hom ~4,741 Discrete
Villa-Garcia MJ~Henry SA, 2011 growth (spot assay) temperature [30°C] SC - Ino + Cho hom ~4,741 Discrete
Villa-Garcia MJ~Henry SA, 2011 growth (spot assay) temperature [37ºC] SC - Ino + Cho hom ~4,741 Discrete

Curation history

Data

Aug. 27, 2018 Waiting for tested.

Tested strains

Aug. 27, 2018 To request.
Sept. 21, 2018 Request sent.
Nov. 29, 2018 Request sent.