Bottoms S~Piotrowski JS, 2018

Pubmed ID 29329531
Title Chemical genomic guided engineering of gamma-valerolactone tolerant yeast.
Authors Scott Bottoms, Quinn Dickinson, Mick McGee, Li Hinchman, Alan Higbee, Alex Hebert, Jose Serate, Dan Xie, Yaoping Zhang, Joshua J Coon, Chad L Myers, Robert Landick, Jeff S Piotrowski
Abstract Gamma valerolactone (GVL) treatment of lignocellulosic bomass is a promising technology for degradation of biomass for biofuel production; however, GVL is toxic to fermentative microbes. Using a combination of chemical genomics with the yeast (Saccharomyces cerevisiae) deletion collection to identify sensitive and resistant mutants, and chemical proteomics to monitor protein abundance in the presence of GVL, we sought to understand the mechanism toxicity and resistance to GVL with the goal of engineering a GVL-tolerant, xylose-fermenting yeast.
Citation Microb. Cell Fact. 2018; 17:5


Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Bottoms S~Piotrowski JS, 2018 growth (pooled culture) gamma-valerolactone [2.3%] YPGal [2%] hap alpha ~4,000 Quantitative only for hits

Curation history

Tested strains

Feb. 8, 2019 Request sent.


Feb. 8, 2019 Request sent.