Kakimoto M~Yoshimura E, 2005

Pubmed ID 16333747
Title Genome-wide screening of aluminum tolerance in Saccharomyces cerevisiae.
Authors Masayuki Kakimoto, Atsushi Kobayashi, Ryouichi Fukuda, Yusuke Ono, Yasuke Ono, Akinori Ohta, Etsuro Yoshimura
Abstract Genome-wide screening has identified 37 Al-tolerance genes in Saccharomyces cerevisiae. These genes can be roughly categorised into three groups on the basis of function, i.e., genes related to vesicle transport processes, signal transduction pathways, and protein mannosylation. The largest group is composed of genes related to vesicle transport processes; severe Al sensitivity was found in yeast strains lacking these genes. The retrograde transport of endosome-derived vesicles back to the Golgi apparatus is an important factor in determining the Al tolerance of the vesicle transport system. The PKC1-MAPK cascade signalling pathway is important in the Al tolerance of signal transduction. The lack of the gene implicated in this process leads to weakened cell wall architecture, rendering the yeast Al-sensitive. Alternatively, Al might attack the cell wall and/or plasma membrane, and, as signalling is prevented in cells devoid of the genes related to signalling processes, the cells may be unable to alleviate the damage. The genes for protein mannosylation are also associated with Al tolerance, demonstrating the importance of cell wall architecture. These genes are involved in cell integrity processes.
Citation Biometals 2005; 18:467-74
Notes Quantitative growth data is provided for the hit in Tables 2-4 of the main PDF. However, the growth of different mutants was measured at different concentrations of Al, thus making them difficult to compare.


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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Kakimoto M~Yoshimura E, 2005 growth (undefined) aluminium trichloride [0.2-1 mM] LPP (low-phosphate, pH 3.5) hap alpha ~4,826 Discrete

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