Yimit A~Brown GW, 2015

Pubmed ID 26163422
Title Genetic Regulation of Dna2 Localization During the DNA Damage Response.
Authors Askar Yimit, Michael Riffle, Grant W Brown
Abstract DNA damage response pathways are crucial for protecting genome stability in all eukaryotes. Saccharomyces cerevisiae Dna2 has both helicase and nuclease activities that are essential for Okazaki fragment maturation, and Dna2 is involved in long-range DNA end resection at double-strand breaks. Dna2 forms nuclear foci in response to DNA replication stress and to double-strand breaks. We find that Dna2-GFP focus formation occurs mainly during S phase in unperturbed cells. Dna2 colocalizes in nuclear foci with 25 DNA repair proteins that define recombination repair centers in response to phleomycin-induced DNA damage. To systematically identify genes that affect Dna2 focus formation, we crossed Dna2-GFP into 4293 nonessential gene deletion mutants and assessed Dna2-GFP nuclear focus formation after phleomycin treatment. We identified 37 gene deletions that affect Dna2-GFP focus formation, 12 with fewer foci and 25 with increased foci. Together these data comprise a useful resource for understanding Dna2 regulation in response to DNA damage.
Citation G3 (Bethesda) 2015; 5:1937-44


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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Yimit A~Brown GW, 2015 DNA repair (Dna2-GFP) phleomycin [5 ug/ml] hap a ~4,293 Discrete

Curation history

Tested strains

Feb. 20, 2019 To request.


Feb. 20, 2019 To request.