Ohya Y~Morishita S, 2005

Pubmed ID 16365294
Title High-dimensional and large-scale phenotyping of yeast mutants.
Authors Yoshikazu Ohya, Jun Sese, Masashi Yukawa, Fumi Sano, Yoichiro Nakatani, Taro L Saito, Ayaka Saka, Tomoyuki Fukuda, Satoru Ishihara, Satomi Oka, Genjiro Suzuki, Machika Watanabe, Aiko Hirata, Miwaka Ohtani, Hiroshi Sawai, Nicolas Fraysse, Jean-Paul Latgé, Jean M François, Markus Aebi, Seiji Tanaka, Sachiko Muramatsu, Hiroyuki Araki, Kintake Sonoike, Satoru Nogami, Shinichi Morishita
Abstract One of the most powerful techniques for attributing functions to genes in uni- and multicellular organisms is comprehensive analysis of mutant traits. In this study, systematic and quantitative analyses of mutant traits are achieved in the budding yeast Saccharomyces cerevisiae by investigating morphological phenotypes. Analysis of fluorescent microscopic images of triple-stained cells makes it possible to treat morphological variations as quantitative traits. Deletion of nearly half of the yeast genes not essential for growth affects these morphological traits. Similar morphological phenotypes are caused by deletions of functionally related genes, enabling a functional assignment of a locus to a specific cellular pathway. The high-dimensional phenotypic analysis of defined yeast mutant strains provides another step toward attributing gene function to all of the genes in the yeast genome.
Citation Proc. Natl. Acad. Sci. U.S.A. 2005; 102:19015-20

Datasets

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Paper Phenotype Condition Reference Collection Tested mutants Data Details
Ohya Y~Morishita S, 2005 Length of the long axis of the nucleus in the daughter cell in budded cells with nuclei in both mother and daughter cells (D177_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Length of the long axis of the nucleus in the mother cell in budded cells with nuclei in both mother and daughter cells (D176_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Length of the short axis of the cell in unbudded cells with single nucleus (C104_A) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Length of the short axis of the daugther cell in budded cells with single nucleus in mother cell or at bud neck (C108_A1B) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Length of the short axis of the daugther cell in budded cells with nuclei in both mother and daughter cells (C108_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Length of the short axis of the mother cell in budded cells with single nucleus in mother cell or at bud neck (C104_A1B) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Length of the short axis of the mother cell in budded cells with nuclei in both mother and daughter cells (C104_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Maximal brightness of nucleus in unbudded cells with single nucleus (D16-1_A) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Maximal brightness of nucleus in budded cells with single nucleus in mother cell or at bud neck (D16-3_A1B) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Maximal brightness of nucleus in budded cells with nuclei in both mother and daughter cells (D16-3_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Maximal brightness of nucleus in daughter in budded cells with nuclei in both mother and daughter cells (D16-2_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Maximal brightness of nucleus in mother in budded cells with nuclei in both mother and daughter cells (D16-1_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Maximal distance between nuclear gravity center and nuclear outline in unbudded cells with single nucleus (D173_A) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Maximal distance between nuclear gravity center and nuclear outline in budded cells with single nucleus in mother cell or at bud neck (D175_A1B) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Maximal distance between nuclear gravity center and nuclear outline in daughter in budded cells with nuclei in both mother and daughter cells (D174_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Maximal distance between nuclear gravity center and nuclear outline in mother in budded cells with nuclei in both mother and daughter cells (D173_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Maximal distance between patches in unbudded cells with single nucleus (A121_A) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Maximal distance between patches in budded cells with single nucleus in mother cell or at bud neck (A121_A1B) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Maximal distance between patches in budded cells with nuclei in both mother and daughter cells (A121_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Maximal intensity of nuclear brightness divided by average in unbudded cells with single nucleus (D194_A) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Maximal intensity of nuclear brightness divided by average in budded cells with single nucleus in mother cell or at bud neck (D196_A1B) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Maximal intensity of nuclear brightness divided by average in budded cells with nuclei in both mother and daughter cells (D196_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Maximal intensity of nuclear brightness divided by average in daughter in budded cells with nuclei in both mother and daughter cells (D195_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Maximal intensity of nuclear brightness divided by average in mother in budded cells with nuclei in both mother and daughter cells (D194_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Mobility of nucleus in daughter in budded cells with nuclei in both mother and daughter cells (D153_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Mobility of nucleus in mother in budded cells with single nucleus in mother cell or at bud neck (D152_A1B) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Mobility of nucleus in mother in budded cells with nuclei in both mother and daughter cells (D152_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Nuclear minimum radius in unbudded cells with single nucleus (D179_A) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Nuclear minimum radius in budded cells with single nucleus in mother cell or at bud neck (D181_A1B) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Nuclear minimum radius in daughter in budded cells with nuclei in both mother and daughter cells (D180_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Nuclear minimum radius in mother in budded cells with nuclei in both mother and daughter cells (D179_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of unbudded cells with single nucleus and delocalized actin (A105_A) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of unbudded cells with delocalized actin (A105) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of budded and unbudded cells with delocalized actin (A111) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of unbudded cells with single nucleus and actin localized at bud site (A106_A) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of unbudded cells with actin localized at bud site (A106) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of budded and unbudded cells with actin localized at bud site, bud tip or in bud (A112) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of budded cells with single nucleus in mother cell or at bud neck and actin localized at bud tip (A107_A1B) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of budded cells with nuclei in both mother and daughter cells and actin localized at bud tip (A107_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of budded cells with actin localized at bud tip (A107) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of budded cells with single nucleus in mother cell or at bud neck and actin localized at bud tip or in bud (A112_A1B) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of budded cells with nuclei in both mother and daughter cells and actin localized at bud tip or in bud (A112_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of budded cells with single nucleus in mother cell or at bud neck and actin localized in the daughter cell (A108_A1B) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of budded cells with nuclei in both mother and daughter cells and actin localized in the daughter cell (A108_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of budded cells with actin localized in the daughter cell (A108) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of budded cells with single nucleus in mother cell or at bud neck and delocalized actin (A109_A1B) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of budded cells with nuclei in both mother and daughter cells and delocalized actin (A109_C) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of budded cells with delocalized actin (A109) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of budded cells with single nucleus in mother cell or at bud neck and actin localized at bud neck (A110_A1B) standard hap a 4,715 Quantitative
Ohya Y~Morishita S, 2005 Number of budded cells with nuclei in both mother and daughter cells and actin localized at bud neck (A110_C) standard hap a 4,715 Quantitative

Curation history

Tested strains

March 12, 2013 Loaded.

Data

March 12, 2013 Loaded.