Andersen MP~Gottschling DE, 2008

Pubmed ID 18562670
Title A genetic screen for increased loss of heterozygosity in Saccharomyces cerevisiae.
Authors Marguerite P Andersen, Zara W Nelson, Elizabeth D Hetrick, Daniel E Gottschling
Abstract Loss of heterozygosity (LOH) can be a driving force in the evolution of mitotic/somatic diploid cells, and cellular changes that increase the rate of LOH have been proposed to facilitate this process. In the yeast Saccharomyces cerevisiae, spontaneous LOH occurs by a number of mechanisms including chromosome loss and reciprocal and nonreciprocal recombination. We performed a screen in diploid yeast to identify mutants with increased rates of LOH using the collection of homozygous deletion alleles of nonessential genes. Increased LOH was quantified at three loci (MET15, SAM2, and MAT) on three different chromosomes, and the LOH events were analyzed as to whether they were reciprocal or nonreciprocal in nature. Nonreciprocal LOH was further characterized as chromosome loss or truncation, a local mutational event (gene conversion or point mutation), or break-induced replication (BIR). The 61 mutants identified could be divided into several groups, including ones that had locus-specific effects. Mutations in genes involved in DNA replication and chromatin assembly led to LOH predominantly via reciprocal recombination. In contrast, nonreciprocal LOH events with increased chromosome loss largely resulted from mutations in genes implicated in kinetochore function, sister chromatid cohesion, or relatively late steps of DNA recombination. Mutants of genes normally involved in early steps of DNA damage repair and signaling produced nonreciprocal LOH without an increased proportion of chromosome loss. Altogether, this study defines a genetic landscape for the basis of increased LOH and the processes by which it occurs.
Citation Genetics 2008; 179:1179-95


Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Andersen MP~Gottschling DE, 2008 genome instability (colony sectoring) standard YPD hom ~4,800 Quantitative only for hits

Curation history

Tested strains

March 8, 2019 To request.
June 28, 2019 Not available.


March 8, 2019 To request.
June 28, 2019 Ready to load.
July 1, 2019 Loaded.