Heuck S~Kanter U, 2010

Pubmed ID 20641020
Title Genome-wide analysis of caesium and strontium accumulation in Saccharomyces cerevisiae.
Authors Sabine Heuck, Udo C Gerstmann, Bernhard Michalke, Ulrike Kanter
Abstract (137)Cs and (90)Sr contribute to significant and long-lasting contamination of the environment with radionuclides. Due to their relatively high biological availability, they are transferred rapidly into biotic systems and may enter the food chain. In this study, we analysed 4862 haploid yeast knockout strains of Saccharomyces cerevisiae to identify genes involved in caesium (Cs(+)) and/or strontium (Sr(2+)) accumulation. According to this analysis, 212 mutant strains were associated with reproducible altered Cs(+) and/or Sr(2+) accumulation. These mutants were deficient for a wide range of cellular processes. Among those, the vacuolar function and biogenesis turned out to be crucial for both Cs(+) and Sr(2+) accumulation. Disruption of the vacuole diminished Cs(+) accumulation, whereas Sr(2+) enrichment was enhanced. Further analysis with a subset of the identified candidates were undertaken comparing the accumulation of Cs(+) and Sr(2+) with their essential counterparts potassium (K(+)) and calcium (Ca(2+)). Sr(2+) and Ca(2+) accumulation was highly correlated in yeast excluding the possibility of a differential regulation or uptake mechanisms. In direct contrast, the respective results suggest that Cs(+) uptake is at least partially dependent on mechanisms distinct from K(+) uptake. Single candidates (e.g. KHA1) are presented which might be specifically responsible for Cs(+) homeostasis.
Citation Yeast 2010; 27:817-35

Datasets

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Heuck S~Kanter U, 2010 abundance of caesium (gamma-counter) caesium chloride [50 uM], Cs-134 [4 kBq/ml] YPD hap a 4,633 Quantitative
Heuck S~Kanter U, 2010 abundance of strontium (gamma-counter) strontium dichloride [50 uM], Sr-85 [4 kBq/ml] YPD hap a 4,633 Quantitative

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