Seeley ES~Eitzen G, 2002

Pubmed ID 11907261
Title Genomic analysis of homotypic vacuole fusion.
Authors E Scott Seeley, Masashi Kato, Nathan Margolis, William Wickner, Gary Eitzen
Abstract Yeast vacuoles undergo fission and homotypic fusion, yielding one to three vacuoles per cell at steady state. Defects in vacuole fusion result in vacuole fragmentation. We have screened 4828 yeast strains, each with a deletion of a nonessential gene, for vacuole morphology defects. Fragmented vacuoles were found in strains deleted for genes encoding known fusion catalysts as well as 19 enzymes of lipid metabolism, 4 SNAREs, 12 GTPases and GTPase effectors, 9 additional known vacuole protein-sorting genes, 16 protein kinases, 2 phosphatases, 11 cytoskeletal proteins, and 28 genes of unknown function. Vacuole fusion and vacuole protein sorting are catalyzed by distinct, but overlapping, sets of proteins. Novel pathways of vacuole priming and docking emerged from this deletion screen. These include ergosterol biosynthesis, phosphatidylinositol (4,5)-bisphosphate turnover, and signaling from Rho GTPases to actin remodeling. These pathways are supported by the sensitivity of the late stages of vacuole fusion to inhibitors of phospholipase C, calcium channels, and actin remodeling. Using databases of yeast protein interactions, we found that many nonessential genes identified in our deletion screen interact with essential genes that are directly involved in vacuole fusion. Our screen reveals regulatory pathways of vacuole docking and provides a genomic basis for studies of this reaction.
Citation Mol. Biol. Cell 2002; 13:782-94
Data abstract Deletion collection was screened for vacuole morphology defects by staining with fluorescent dye FM4-64.

Datasets

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Seeley ES~Eitzen G, 2002 vacuole fragmented (FM 4-64 dye) standard YPD + G418 [20 ug/ml] hap a/hap alpha/hom ~4,828 Discrete
Seeley ES~Eitzen G, 2002 vacuole enlarged (FM 4-64 dye) standard YPD + G418 [20 ug/ml] hap a/hap alpha/hom ~4,828 Discrete
Seeley ES~Eitzen G, 2002 vacuole class E (FM 4-64 dye) standard YPD + G418 [20 ug/ml] hap a/hap alpha/hom ~4,828 Discrete
Seeley ES~Eitzen G, 2002 multiple small vacuoles (FM 4-64 dye) standard YPD + G418 [20 ug/ml] hap a/hap alpha/hom ~4,828 Discrete

Curation history

Tested strains

March 24, 2020 To request.
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Data

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