Mattiazzi Usaj M~Andrews BJ, 2020

Pubmed ID 32064787
Title Systematic genetics and single-cell imaging reveal widespread morphological pleiotropy and cell-to-cell variability.
Authors Mojca Mattiazzi Usaj, Nil Sahin, Helena Friesen, Carles Pons, Matej Usaj, Myra Paz D Masinas, Ermira Shuteriqi, Aleksei Shkurin, Patrick Aloy, Quaid Morris, Charles Boone, Brenda J Andrews
Abstract Our ability to understand the genotype-to-phenotype relationship is hindered by the lack of detailed understanding of phenotypes at a single-cell level. To systematically assess cell-to-cell phenotypic variability, we combined automated yeast genetics, high-content screening and neural network-based image analysis of single cells, focussing on genes that influence the architecture of four subcellular compartments of the endocytic pathway as a model system. Our unbiased assessment of the morphology of these compartments-endocytic patch, actin patch, late endosome and vacuole-identified 17 distinct mutant phenotypes associated with ~1,600 genes (~30% of all yeast genes). Approximately half of these mutants exhibited multiple phenotypes, highlighting the extent of morphological pleiotropy. Quantitative analysis also revealed that incomplete penetrance was prevalent, with the majority of mutants exhibiting substantial variability in phenotype at the single-cell level. Our single-cell analysis enabled exploration of factors that contribute to incomplete penetrance and cellular heterogeneity, including replicative age, organelle inheritance and response to stress.
Citation Mol. Syst. Biol. 2020; 16:e9243

Datasets

Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Mattiazzi Usaj M~Andrews BJ, 2020 actin aggregate (Sac6) standard SD[low-fluor] + Met + Leu + Ura + NAT + G418 hap a 4,471 Quantitative
Mattiazzi Usaj M~Andrews BJ, 2020 actin bright patches (Sac6) standard SD[low-fluor] + Met + Leu + Ura + NAT + G418 hap a 4,471 Quantitative
Mattiazzi Usaj M~Andrews BJ, 2020 actin decreased patch number (Sac6) standard SD[low-fluor] + Met + Leu + Ura + NAT + G418 hap a 4,471 Quantitative
Mattiazzi Usaj M~Andrews BJ, 2020 actin depolarized patches (Sac6) standard SD[low-fluor] + Met + Leu + Ura + NAT + G418 hap a 4,471 Quantitative
Mattiazzi Usaj M~Andrews BJ, 2020 coat aggregate (Sla1) standard SD[low-fluor] + Met + Leu + Ura + NAT + G418 hap a 4,531 Quantitative
Mattiazzi Usaj M~Andrews BJ, 2020 coat decreased patch number (Sla1) standard SD[low-fluor] + Met + Leu + Ura + NAT + G418 hap a 4,531 Quantitative
Mattiazzi Usaj M~Andrews BJ, 2020 coat depolarized patches (Sla1) standard SD[low-fluor] + Met + Leu + Ura + NAT + G418 hap a 4,531 Quantitative
Mattiazzi Usaj M~Andrews BJ, 2020 coat increased patch number (Sla1) standard SD[low-fluor] + Met + Leu + Ura + NAT + G418 hap a 4,531 Quantitative
Mattiazzi Usaj M~Andrews BJ, 2020 late endosome fragmented (Snf7) standard SD[low-fluor] + Met + Leu + Ura + NAT + G418 hap a 4,446 Quantitative
Mattiazzi Usaj M~Andrews BJ, 2020 late endosome membrane (Snf7) standard SD[low-fluor] + Met + Leu + Ura + NAT + G418 hap a 4,446 Quantitative
Mattiazzi Usaj M~Andrews BJ, 2020 late endosome condensed (Snf7) standard SD[low-fluor] + Met + Leu + Ura + NAT + G418 hap a 4,446 Quantitative
Mattiazzi Usaj M~Andrews BJ, 2020 vacuole class E (Vph1) standard SD[low-fluor] + Met + Leu + Ura + NAT + G418 hap a 4,523 Quantitative
Mattiazzi Usaj M~Andrews BJ, 2020 vacuole enlarged (Vph1) standard SD[low-fluor] + Met + Leu + Ura + NAT + G418 hap a 4,523 Quantitative
Mattiazzi Usaj M~Andrews BJ, 2020 vacuole vATPase defect (Vph1) standard SD[low-fluor] + Met + Leu + Ura + NAT + G418 hap a 4,523 Quantitative
Mattiazzi Usaj M~Andrews BJ, 2020 vacuole fragmented (Vph1) standard SD[low-fluor] + Met + Leu + Ura + NAT + G418 hap a 4,523 Quantitative
Mattiazzi Usaj M~Andrews BJ, 2020 vacuole class G (Vph1) standard SD[low-fluor] + Met + Leu + Ura + NAT + G418 hap a 4,523 Quantitative
Mattiazzi Usaj M~Andrews BJ, 2020 vacuole multilobed (Vph1) standard SD[low-fluor] + Met + Leu + Ura + NAT + G418 hap a 4,523 Quantitative

Curation history

Data

March 25, 2020 Ready to load.
May 27, 2020 Loaded.

Tested strains

March 25, 2020 Ready to load.
May 27, 2020 Loaded.