Yang J~Nystrom T, 2016

Pubmed ID 27033550
Title Spatial sequestration and detoxification of Huntingtin by the ribosome quality control complex.
Authors Junsheng Yang, Xinxin Hao, Xiuling Cao, Beidong Liu, Thomas Nyström
Abstract Huntington disease (HD) is a neurological disorder caused by polyglutamine expansions in mutated Huntingtin (mHtt) proteins, rendering them prone to form inclusion bodies (IB). We report that in yeast, such IB formation is a factor-dependent process subjected to age-related decline. A genome-wide, high-content imaging approach, identified the E3 ubiquitin ligase, Ltn1 of the ribosome quality control complex (RQC) as a key factor required for IB formation, ubiquitination, and detoxification of model mHtt. The failure of ltn1∆ cells to manage mHtt was traced to another RQC component, Tae2, and inappropriate control of heat shock transcription factor, Hsf1, activity. Moreover, super-resolution microscopy revealed that mHtt toxicity in RQC-deficient cells was accompanied by multiple mHtt aggregates altering actin cytoskeletal structures and retarding endocytosis. The data demonstrates that spatial sequestration of mHtt into IBs is policed by the RQC-Hsf1 regulatory system and that such compartmentalization, rather than ubiquitination, is key to mHtt detoxification.
Citation Elife 2016; 5:.


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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Yang J~Nystrom T, 2016 expression of mutant huntingtin fragment [GAL] SC - Ura, Gal [2%], Raf [2%] hap a (post-SGA) N/A Quantitative only for hits

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