Mir-Rashed N~Smith ML, 2010

Pubmed ID 20429770
Title Disruption of fungal cell wall by antifungal Echinacea extracts.
Authors Nadereh Mir-Rashed, Isabel Cruz, Matthew Jessulat, Michel Dumontier, Claire Chesnais, Juliana Ng, Virginie Treyvaud Amiguet, Ashkan Golshani, John T Arnason, Myron L Smith
Abstract In addition to widespread use in reducing the symptoms of colds and flu, Echinacea is traditionally employed to treat fungal and bacterial infections. However, to date the mechanism of antimicrobial activity of Echinacea extracts remains unclear. We utilized a set of ∼4,600 viable gene deletion mutants of Saccharomyces cerevisiae to identify mutations that increase sensitivity to Echinacea. Thus, a set of chemical-genetic profiles for 16 different Echinacea treatments was generated, from which a consensus set of 23 Echinacea-sensitive mutants was identified. Of the 23 mutants, only 16 have a reported function. Ten of these 16 are involved in cell wall integrity/structure suggesting that a target for Echinacea is the fungal cell wall. Follow-up analyses revealed an increase in sonication-associated cell death in the yeasts S. cerevisiae and Cryptococcus neoformans after Echinacea extract treatments. Furthermore, fluorescence microscopy showed that Echinacea-treated S. cerevisiae was significantly more prone to cell wall damage than non-treated cells. This study further demonstrates the potential of gene deletion arrays to understand natural product antifungal mode of action and provides compelling evidence that the fungal cell wall is a target of Echinacea extracts and may thus explain the utility of this phytomedicine in treating mycoses.
Citation Med Mycol 2010; 48:949-58
Data abstract Deletion collection was tested for growth on Echinacea extract.

Datasets

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Mir-Rashed N~Smith ML, 2010 growth (colony size) SG1 E. purpurea extract [5.2 mg/mL] YPD hap a ~4,600 Quantitative only for hits
Mir-Rashed N~Smith ML, 2010 growth (colony size) SG1 E. purpurea extract [4.5 mg/mL], UV [10 W/m2], UV [10 W/m2], UV [10 W/m2] YPD hap a ~4,600 Quantitative only for hits
Mir-Rashed N~Smith ML, 2010 growth (colony size) SGEPR E. purpurea extract [4.5 mg/mL] YPD hap a ~4,600 Quantitative only for hits
Mir-Rashed N~Smith ML, 2010 growth (colony size) SGEPR E. purpurea extract [2.3 mg/mL], UV [10 W/m2], UV [10 W/m2], UV [10 W/m2] YPD hap a ~4,600 Quantitative only for hits
Mir-Rashed N~Smith ML, 2010 growth (colony size) SGEPF E. purpurea extract [1.3 mg/mL] YPD hap a ~4,600 Quantitative only for hits
Mir-Rashed N~Smith ML, 2010 growth (colony size) SGEPF E. purpurea extract [0.8 mg/mL], UV [10 W/m2], UV [10 W/m2], UV [10 W/m2] YPD hap a ~4,600 Quantitative only for hits
Mir-Rashed N~Smith ML, 2010 growth (colony size) SGEPH E. purpurea extract [2.1 mg/mL] YPD hap a ~4,600 Quantitative only for hits
Mir-Rashed N~Smith ML, 2010 growth (colony size) SGEPH E. purpurea extract [0.8 mg/mL], UV [10 W/m2], UV [10 W/m2], UV [10 W/m2] YPD hap a ~4,600 Quantitative only for hits
Mir-Rashed N~Smith ML, 2010 growth (colony size) SGEPLS E. purpurea extract [4.0 mg/mL] YPD hap a ~4,600 Quantitative only for hits
Mir-Rashed N~Smith ML, 2010 growth (colony size) SGEPLS E. purpurea extract [2.1 mg/mL], UV [10 W/m2], UV [10 W/m2], UV [10 W/m2] YPD hap a ~4,600 Quantitative only for hits
Mir-Rashed N~Smith ML, 2010 growth (colony size) SG7a E. pallida extract [1.8 mg/mL] YPD hap a ~4,600 Quantitative only for hits
Mir-Rashed N~Smith ML, 2010 growth (colony size) SG7a E. pallida extract [1.8 mg/mL], UV [10 W/m2], UV [10 W/m2], UV [10 W/m2] YPD hap a ~4,600 Quantitative only for hits
Mir-Rashed N~Smith ML, 2010 growth (colony size) SGEARa E. pallida extract [0.6 mg/mL] YPD hap a ~4,600 Quantitative only for hits
Mir-Rashed N~Smith ML, 2010 growth (colony size) SGEARa E. pallida extract [0.6 mg/mL], UV [10 W/m2], UV [10 W/m2], UV [10 W/m2] YPD hap a ~4,600 Quantitative only for hits
Mir-Rashed N~Smith ML, 2010 growth (colony size) SGEARb E. pallida extract [1.3 mg/mL] YPD hap a ~4,600 Quantitative only for hits
Mir-Rashed N~Smith ML, 2010 growth (colony size) SGEARb E. pallida extract [0.3 mg/mL], UV [10 W/m2], UV [10 W/m2], UV [10 W/m2] YPD hap a ~4,600 Quantitative only for hits

Curation history

Tested strains

June 4, 2020 To request.
June 29, 2020 Request sent.
July 16, 2020 Not available.
July 16, 2020 Request sent.

Data

June 4, 2020 To request.
June 29, 2020 Request sent.
July 16, 2020 Ready to load.
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July 16, 2020 Request sent.
Aug. 26, 2020 Loaded.