Minear S~Cyert MS, 2011

Pubmed ID 21908599
Title Curcumin inhibits growth of Saccharomyces cerevisiae through iron chelation.
Authors Steven Minear, Allyson F O'Donnell, Anna Ballew, Guri Giaever, Corey Nislow, Tim Stearns, Martha S Cyert
Abstract Curcumin, a polyphenol derived from turmeric, is an ancient therapeutic used in India for centuries to treat a wide array of ailments. Interest in curcumin has increased recently, with ongoing clinical trials exploring curcumin as an anticancer therapy and as a protectant against neurodegenerative diseases. In vitro, curcumin chelates metal ions. However, although diverse physiological effects have been documented for this compound, curcumin's mechanism of action on mammalian cells remains unclear. This study uses yeast as a model eukaryotic system to dissect the biological activity of curcumin. We found that yeast mutants lacking genes required for iron and copper homeostasis are hypersensitive to curcumin and that iron supplementation rescues this sensitivity. Curcumin penetrates yeast cells, concentrates in the endoplasmic reticulum (ER) membranes, and reduces the intracellular iron pool. Curcumin-treated, iron-starved cultures are enriched in unbudded cells, suggesting that the G(1) phase of the cell cycle is lengthened. A delay in cell cycle progression could, in part, explain the antitumorigenic properties associated with curcumin. We also demonstrate that curcumin causes a growth lag in cultured human cells that is remediated by the addition of exogenous iron. These findings suggest that curcumin-induced iron starvation is conserved from yeast to humans and underlies curcumin's medicinal properties.
Citation Eukaryot Cell 2011; 10:1574-81
Data abstract Pooled deletion collection was tested for growth on curcumin.

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Minear S~Cyert MS, 2011 growth (pooled culture, microarray) curcumin [150 uM], time [5 gen] YPD hom ~4,700 Quantitative only for hits

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