|Title||Screening the budding yeast genome reveals unique factors affecting K2 toxin susceptibility.|
|Authors||Elena Servienė, Juliana Lukša, Irma Orentaitė, Denis L J Lafontaine, Jaunius Urbonavičius|
|Abstract||Understanding how biotoxins kill cells is of prime importance in biomedicine and the food industry. The budding yeast (S. cerevisiae) killers serve as a convenient model to study the activity of biotoxins consistently supplying with significant insights into the basic mechanisms of virus-host cell interactions and toxin entry into eukaryotic target cells. K1 and K2 toxins are active at the cell wall, leading to the disruption of the plasma membrane and subsequent cell death by ion leakage. K28 toxin is active in the cell nucleus, blocking DNA synthesis and cell cycle progression, thereby triggering apoptosis. Genome-wide screens in the budding yeast S. cerevisiae identified several hundred effectors of K1 and K28 toxins. Surprisingly, no such screen had been performed for K2 toxin, the most frequent killer toxin among industrial budding yeasts.|
|Citation||PLoS ONE 2012; 7:e50779|
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