Servienė E~Urbonavičius J, 2012

Pubmed ID 23227207
Title Screening the budding yeast genome reveals unique factors affecting K2 toxin susceptibility.
Authors Elena Servienė, Juliana Lukša, Irma Orentaitė, Denis L J Lafontaine, Jaunius Urbonavičius
Abstract Understanding how biotoxins kill cells is of prime importance in biomedicine and the food industry. The budding yeast (S. cerevisiae) killers serve as a convenient model to study the activity of biotoxins consistently supplying with significant insights into the basic mechanisms of virus-host cell interactions and toxin entry into eukaryotic target cells. K1 and K2 toxins are active at the cell wall, leading to the disruption of the plasma membrane and subsequent cell death by ion leakage. K28 toxin is active in the cell nucleus, blocking DNA synthesis and cell cycle progression, thereby triggering apoptosis. Genome-wide screens in the budding yeast S. cerevisiae identified several hundred effectors of K1 and K28 toxins. Surprisingly, no such screen had been performed for K2 toxin, the most frequent killer toxin among industrial budding yeasts.
Citation PLoS ONE 2012; 7:e50779
Data abstract Deletion collection tested for sensitivity/resistance to K2 toxin.

Datasets

Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Servienė E~Urbonavičius J, 2012 growth (colony size) K2 killer toxin [secreted from lawn (2x10^5)] MBA hap a ~4,774 Discrete
Servienė E~Urbonavičius J, 2012 growth (colony size) K2 killer toxin [secreted from lawn (2x10^6)] MBA hap a ~4,774 Discrete
Servienė E~Urbonavičius J, 2012 growth (colony size) K2 killer toxin [300 U/plate] MBA hap a ~4,774 Discrete
Servienė E~Urbonavičius J, 2012 growth (colony size) K2 killer toxin [600 U/plate] MBA hap a ~4,774 Discrete

Curation history

Data

Aug. 5, 2020 To request.

Tested strains

July 4, 2020 To request.