Darvishi E~Smith ML, 2013

Pubmed ID 23718894
Title Thymol antifungal mode of action involves telomerase inhibition.
Authors Emad Darvishi, Mansoor Omidi, Ali Akbar Bushehri, Ashkan Golshani, Myron L Smith
Abstract The antifungal mode of action of thymol was investigated by a chemical-genetic profile analysis. Growth of each of ~4700 haploid Saccharomyces cerevisiae gene deletion mutants was monitored on medium with a subinhibitory concentration (50 μg/ml) of thymol and compared to growth on non-thymol control medium. This analysis revealed that, of the 76 deletion mutants with the greatest degree of susceptibility to thymol, 29% had deletions in genes involved in telomere length maintenance. A telomere restriction fragment (TRF) length assay showed that yeast exposed to a subinhibitory concentration of thymol for 15 days had telomere size reductions of 13-20% compared to non-thymol controls. By accelerating telomere shortening, thymol may increase the rate of cell senescence and apoptosis. Furthermore, real-time RT-PCR analysis revealed approximately two-fold reductions in EST2 mRNA but no change in TLC1 RNA in thymol-treated S. cerevisiae relative to untreated cells. EST2 encodes the essential reverse transcriptase subunit of telomerase that uses TLC1 RNA as a template during addition of TG(1-3) repeats to maintain telomere ends. This study provides compelling evidence that a primary mode of thymol antifungal activity is through inhibition of transcription of EST2 and thus telomerase activity.
Citation Med Mycol 2013; 51:826-34

Datasets

Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Darvishi E~Smith ML, 2013 growth (colony size) thymol [50 ug/ml] YPD hap a ~4,700 None

Curation history

Tested strains

June 29, 2020 To request.
July 17, 2020 Request sent.
Sept. 21, 2020 Request sent.

Data

June 29, 2020 To request.
July 17, 2020 Request sent.
Sept. 21, 2020 Request sent.