Garcia R~Arroyo J, 2015

Pubmed ID 26341223
Title Genomic profiling of fungal cell wall-interfering compounds: identification of a common gene signature.
Authors Raúl García, Javier Botet, José Manuel Rodríguez-Peña, Clara Bermejo, Juan Carlos Ribas, José Luis Revuelta, César Nombela, Javier Arroyo
Abstract The fungal cell wall forms a compact network whose integrity is essential for cell morphology and viability. Thus, fungal cells have evolved mechanisms to elicit adequate adaptive responses when cell wall integrity (CWI) is compromised. Functional genomic approaches provide a unique opportunity to globally characterize these adaptive mechanisms. To provide a global perspective on these CWI regulatory mechanisms, we developed chemical-genomic profiling of haploid mutant budding yeast cells to systematically identify in parallel those genes required to cope with stresses interfering the cell wall by different modes of action: β-1,3 glucanase and chitinase activities (zymolyase), inhibition of β-1,3 glucan synthase (caspofungin) and binding to chitin (Congo red).
Citation BMC Genomics 2015; 16:683

Datasets

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Garcia R~Arroyo J, 2015 growth (culture turbidity) zymolyase [30 U/ml] YPD hap a 4,762 Quantitative
Garcia R~Arroyo J, 2015 growth (spot assay) Congo red [50 ug/ml], Congo red [50 ug/ml] YPD hap a 4,765 Discrete
Garcia R~Arroyo J, 2015 growth (culture turbidity) caspofungin [10-30 ng/ml], caspofungin [10-30 ng/ml] YPD hap a 4,765 Discrete
Garcia R~Arroyo J, 2015 growth (culture turbidity) caspofungin [60-120 ng/ml], caspofungin [60-120 ng/ml] YPD hap a 4,765 Discrete

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