Luo C~Jiang L, 2016

Pubmed ID 26994103
Title The endosomal sorting complex required for transport (ESCRT) is required for the sensitivity of yeast cells to nickel ions in Saccharomyces cerevisiae.
Authors Chong Luo, Chunlei Cao, Linghuo Jiang
Abstract Nickel is one of the toxic environment metal pollutants and is linked to various human diseases. In this study, through a functional genomics approach we have identified 16 nickel-sensitive and 22 nickel-tolerant diploid deletion mutants of budding yeast genes, many of which are novel players in the regulation of nickel homeostasis. The 16 nickel-sensitive mutants are of genes mainly involved in the protein folding, modification and destination and the cellular transport processes, while the 22 nickel-tolerant mutants are of genes encoding components of ESCRT complexes as well as protein factors involved in both the cell wall integrity maintenance and the vacuolar protein sorting process. In consistence with their phenotypes, most of these nickel-sensitive mutants show reduced intracellular nickel contents, while the majority of these nickel-tolerant mutants show elevated intracellular nickel contents, as compared to the wild type in response to nickel stress. Our data provides a basis for our understanding the regulation of nickel homeostasis and molecular mechanisms of nickel-induced human pathogenesis.
Citation FEMS Yeast Res. 2016; 16:.

Datasets

Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Luo C~Jiang L, 2016 growth (colony size) nickel sulfate [0.3 mM] YPD hom 4,717 Discrete
Luo C~Jiang L, 2016 growth (colony size) nickel sulfate [0.6 mM] YPD hom 4,717 Discrete

Curation history

Data

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Tested strains

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