Alfatah M~Arumugam P, 2019

Pubmed ID 30679518
Title Hypoculoside, a sphingoid base-like compound from Acremonium disrupts the membrane integrity of yeast cells.
Authors Mohammad Alfatah, Jin Huei Wong, Choy Eng Nge, Kiat Whye Kong, Kia Ngee Low, Chung Yan Leong, Sharon Crasta, Madhaiyan Munusamy, Amanda Mun Leng Chang, Shawn Hoon, Siew Bee Ng, Yoganathan Kanagasundaram, Prakash Arumugam
Abstract We have isolated Hypoculoside, a new glycosidic amino alcohol lipid from the fungus Acremonium sp. F2434 belonging to the order Hypocreales and determined its structure by 2D-NMR (Nuclear Magnetic Resonance) spectroscopy. Hypoculoside has antifungal, antibacterial and cytotoxic activities. Homozygous profiling (HOP) of hypoculoside in Saccharomyces cerevisiae (budding yeast) revealed that several mutants defective in vesicular trafficking and vacuolar protein transport are sensitive to hypoculoside. Staining of budding yeast cells with the styryl dye FM4-64 indicated that hypoculoside damaged the vacuolar structure. Furthermore, the propidium iodide (PI) uptake assay showed that hypoculoside disrupted the plasma membrane integrity of budding yeast cells. Interestingly, the glycosidic moiety of hypoculoside is required for its deleterious effect on growth, vacuoles and plasma membrane of budding yeast cells.
Citation Sci Rep 2019; 9:710


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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Alfatah M~Arumugam P, 2019 growth (pooled culture, barseq) hypoculoside [2.7 uM] YPD, HEPES [25 mM], pH 6.8, DMSO hom 3,683 Quantitative

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