Tun NM~Wu MJ, 2014

Pubmed ID 24926745
Title Identification of aluminium transport-related genes via genome-wide phenotypic screening of Saccharomyces cerevisiae.
Authors Nay M Tun, Patrick J O'Doherty, Zhong-Hua Chen, Xi-Yang Wu, Trevor D Bailey, Cindy Kersaitis, Ming J Wu
Abstract Genome-wide screening using gene deletion mutants has been widely carried out with numerous toxicants including oxidants and metal ions. The focus of such studies usually centres on identifying sensitive phenotypes against a given toxicant. Here, we screened the complete collection of yeast gene deletion mutants (5047) with increasing concentrations of aluminium sulphate (0.4, 0.8, 1.6 and 3.2 mM) in order to discover aluminium (Al(3+)) tolerant phenotypes. Fifteen genes were found to be associated with Al(3+) transport because their deletion mutants exhibited Al(3+) tolerance, including lem3Δ, hal5Δ and cka2Δ. Deletion of CKA2, a catalytic subunit of tetrameric protein kinase CK2, gives rise to the most pronounced resistance to Al(3+) by showing significantly higher growth compared to the wild type. Functional analysis revealed that both molecular regulation and endocytosis are involved in Al(3+) transport for yeast. Further investigations were extended to all the four subunits of CK2 (CKA1, CKA2, CKB1 and CKB2) and the other 14 identified mutants under a spectrum of metal ions, including Al(3+), Zn(2+), Mn(2+), Fe(2+), Fe(3+), Co(3+), Ga(3+), Cd(2+), In(3+), Ni(2+) and Cu(2+), as well as hydrogen peroxide and diamide, in order to unravel cross-tolerance amongst metal ions and the effect of the oxidants. Finally, the implication of the findings in Al(3+) transport for the other species like plants and humans is discussed.
Citation Metallomics 2014; 6:1558-64

Datasets

Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Tun NM~Wu MJ, 2014 growth (culture turbidity) aluminium sulfate (anhydrous) [1.6 mM], aluminium sulfate (anhydrous) [1.6 mM] SC hom 4,873 Quantitative
Tun NM~Wu MJ, 2014 growth (culture turbidity) aluminium sulfate (anhydrous) [3.2 mM], aluminium sulfate (anhydrous) [3.2 mM] SC hom 4,873 Quantitative
Tun NM~Wu MJ, 2014 growth (culture turbidity) standard SC hom 4,873 Quantitative

Curation history

Data

June 11, 2020 Ready to load.
June 23, 2020 Loaded.

Tested strains

June 11, 2020 Ready to load.
June 23, 2020 Loaded.