Dunn CD~Jensen RE, 2006

Pubmed ID 16267274
Title A genomewide screen for petite-negative yeast strains yields a new subunit of the i-AAA protease complex.
Authors Cory D Dunn, Marina S Lee, Forrest A Spencer, Robert E Jensen
Abstract Unlike many other organisms, the yeast Saccharomyces cerevisiae can tolerate the loss of mitochondrial DNA (mtDNA). Although a few proteins have been identified that are required for yeast cell viability without mtDNA, the mechanism of mtDNA-independent growth is not completely understood. To probe the relationship between the mitochondrial genome and cell viability, we conducted a microarray-based, genomewide screen for mitochondrial DNA-dependent yeast mutants. Among the several genes that we discovered is MGR1, which encodes a novel subunit of the i-AAA protease complex located in the mitochondrial inner membrane. mgr1Delta mutants retain some i-AAA protease activity, yet mitochondria lacking Mgr1p contain a misassembled i-AAA protease and are defective for turnover of mitochondrial inner membrane proteins. Our results highlight the importance of the i-AAA complex and proteolysis at the inner membrane in cells lacking mitochondrial DNA.
Citation Mol. Biol. Cell 2006; 17:213-26


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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Dunn CD~Jensen RE, 2006 growth (pooled culture) ethidium bromide [25 ug/ml] YPD hap a 3,759 Quantitative

Curation history

Tested strains

March 6, 2013 Loaded.


March 6, 2013 Loaded.