Lockshon D~Kennedy BK, 2007

Pubmed ID 17151231
Title The sensitivity of yeast mutants to oleic acid implicates the peroxisome and other processes in membrane function.
Authors Daniel Lockshon, Lauren E Surface, Emily O Kerr, Matt Kaeberlein, Brian K Kennedy
Abstract The peroxisome, sole site of beta-oxidation in Saccharomyces cerevisiae, is known to be required for optimal growth in the presence of fatty acid. Screening of the haploid yeast deletion collection identified approximately 130 genes, 23 encoding peroxisomal proteins, necessary for normal growth on oleic acid. Oleate slightly enhances growth of wild-type yeast and inhibits growth of all strains identified by the screen. Nonperoxisomal processes, among them chromatin modification by H2AZ, Pol II mediator function, and cell-wall-associated activities, also prevent oleate toxicity. The most oleate-inhibited strains lack Sap190, a putative adaptor for the PP2A-type protein phosphatase Sit4 (which is also required for normal growth on oleate) and Ilm1, a protein of unknown function. Palmitoleate, the other main unsaturated fatty acid of Saccharomyces, fails to inhibit growth of the sap190delta, sit4delta, and ilm1delta strains. Data that suggest that oleate inhibition of the growth of a peroxisomal mutant is due to an increase in plasma membrane porosity are presented. We propose that yeast deficient in peroxisomal and other functions are sensitive to oleate perhaps because of an inability to effectively control the fatty acid composition of membrane phospholipids.
Citation Genetics 2007; 175:77-91

Datasets

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Paper Phenotype Condition Reference Collection Tested mutants Data Details
Lockshon D~Kennedy BK, 2007 growth (colony size) oleic acid [0.1%] hap alpha ~4,773 Discrete

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