Michelsen K~Schwappach B, 2007

Pubmed ID 17954604
Title Novel cargo-binding site in the beta and delta subunits of coatomer.
Authors Kai Michelsen, Volker Schmid, Jutta Metz, Katja Heusser, Urban Liebel, Torsten Schwede, Anne Spang, Blanche Schwappach
Abstract Arginine (R)-based ER localization signals are sorting motifs that confer transient ER localization to unassembled subunits of multimeric membrane proteins. The COPI vesicle coat binds R-based signals but the molecular details remain unknown. Here, we use reporter membrane proteins based on the proteolipid Pmp2 fused to GFP and allele swapping of COPI subunits to map the recognition site for R-based signals. We show that two highly conserved stretches--in the beta- and delta-COPI subunits--are required to maintain Pmp2GFP reporters exposing R-based signals in the ER. Combining a deletion of 21 residues in delta-COP together with the mutation of three residues in beta-COP gave rise to a COPI coat that had lost its ability to recognize R-based signals, whilst the recognition of C-terminal di-lysine signals remained unimpaired. A homology model of the COPI trunk domain illustrates the recognition of R-based signals by COPI.
Citation J. Cell Biol. 2007; 179:209-17

Datasets

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Michelsen K~Schwappach B, 2007 transport to/from ER (Pmp2-LRKR) standard hap a N/A Discrete
Michelsen K~Schwappach B, 2007 transport to/from ER (Pmp2-LRKR) standard tet N/A Discrete

Curation history

Tested strains

March 19, 2014 Request sent.
Nov. 11, 2014 Request sent.
Jan. 28, 2015 Request abandoned.

Data

March 19, 2014 Waiting for tested.
Jan. 28, 2015 Ready to load.
April 21, 2017 Loaded.
April 21, 2017 Loaded.