Rothfels K~Segall J, 2005

Pubmed ID 16024810
Title Components of the ESCRT pathway, DFG16, and YGR122w are required for Rim101 to act as a corepressor with Nrg1 at the negative regulatory element of the DIT1 gene of Saccharomyces cerevisiae.
Authors Karen Rothfels, Jason C Tanny, Enikö Molnar, Helena Friesen, Cosimo Commisso, Jacqueline Segall
Abstract The divergently transcribed DIT1 and DIT2 genes of Saccharomyces cerevisiae, which belong to the mid-late class of sporulation-specific genes, are subject to Ssn6-Tup1-mediated repression in mitotic cells. The Ssn6-Tup1 complex, which is required for repression of diverse sets of coordinately regulated genes, is known to be recruited to target genes by promoter-specific DNA-binding proteins. In this study, we show that a 42-bp negative regulatory element (NRE) present in the DIT1-DIT2 intergenic region consists of two distinct subsites and that a multimer of each subsite supports efficient Ssn6-Tup1-dependent repression of a CYC1-lacZ reporter gene. By genetic screening procedures, we identified DFG16, YGR122w, VPS36, and the DNA-binding proteins Rim101 and Nrg1 as potential mediators of NRE-directed repression. We show that Nrg1 and Rim101 bind simultaneously to adjacent target sites within the NRE in vitro and act as corepressors in vivo. We have found that the ability of Rim101 to be proteolytically processed to its active form and mediate NRE-directed repression not only depends on the previously characterized RIM signaling pathway but also requires Dfg16, Ygr122w, and components of the ESCRT trafficking pathway. Interestingly, Rim101 was processed in bro1 and doa4 strains but was unable to mediate efficient repression.
Citation Mol. Cell. Biol. 2005; 25:6772-88
Data abstract Deletion collection was screened for genes that contribute to transcriptional repression of a negative regulatory element present in the intergenic region of DIT1 and DIT2.

Datasets

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Rothfels K~Segall J, 2005 gene expression (CYC1-NRE42-lacZ) standard hap a (post-SGA) N/A Unknown
Rothfels K~Segall J, 2005 gene expression (CYC1-NRE25-lacZ) standard hap a (post-SGA) N/A Unknown

Curation history

Tested strains

Sept. 14, 2020 To request.
Sept. 18, 2020 Request sent.

Data

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Sept. 18, 2020 Request sent.