Wilson MA~van Hoof A, 2007

Pubmed ID 17660569
Title A genomic screen in yeast reveals novel aspects of nonstop mRNA metabolism.
Authors Marenda A Wilson, Stacie Meaux, Ambro van Hoof
Abstract Nonstop mRNA decay, a specific mRNA surveillance pathway, rapidly degrades transcripts that lack in-frame stop codons. The cytoplasmic exosome, a complex of 3'-5' exoribonucleases involved in RNA degradation and processing events, degrades nonstop transcripts. To further understand how nonstop mRNAs are recognized and degraded, we performed a genomewide screen for nonessential genes that are required for nonstop mRNA decay. We identified 16 genes that affect the expression of two different nonstop reporters. Most of these genes affected the stability of a nonstop mRNA reporter. Additionally, three mutations that affected nonstop gene expression without stabilizing nonstop mRNA levels implicated the proteasome. This finding not only suggested that the proteasome may degrade proteins encoded by nonstop mRNAs, but also supported previous observations that rapid decay of nonstop mRNAs cannot fully explain the lack of the encoded proteins. Further, we show that the proteasome and Ski7p affected expression of nonstop reporter genes independently of each other. In addition, our results implicate inositol 1,3,4,5,6-pentakisphosphate as an inhibitor of nonstop mRNA decay.
Citation Genetics 2007; 177:773-84


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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Wilson MA~van Hoof A, 2007 growth (undefined) standard SC - His hap a 4,785 Discrete

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