Galvan IJ~Smith ML, 2008

Pubmed ID 17950387
Title Antifungal and antioxidant activities of the phytomedicine pipsissewa, Chimaphila umbellata.
Authors Imelda J Galván, Nadereh Mir-Rashed, Matthew Jessulat, Monica Atanya, Ashkan Golshani, Tony Durst, Philippe Petit, Virginie Treyvaud Amiguet, Teun Boekhout, Richard Summerbell, Isabel Cruz, John T Arnason, Myron L Smith
Abstract Bioassay-guided fractionation of Chimaphila umbellata (L.) W. Bart (Pyrolaceae) ethanol extracts led to the identification of 2,7-dimethyl-1,4-naphthoquinone (chimaphilin) as the principal antifungal component. The structure of chimaphilin was confirmed by 1H and 13C NMR spectroscopy. The antifungal activity of chimaphilin was evaluated using the microdilution method with Saccharomyces cerevisiae (0.05mg/mL) and the dandruff-associated fungi Malassezia globosa (0.39mg/mL) and Malassezia restricta (0.55mg/mL). Pronounced antioxidant activity of C. umbellata crude extract was also identified using the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, suggesting this phytomedicine has an antioxidant function in wound healing. A chemical-genetic profile was completed with chimaphilin using approximately 4700 S. cerevisiae gene deletion mutants. Cellular roles of deleted genes in the most susceptible mutants and secondary assays indicate that the targets for chimaphilin include pathways involved in cell wall biogenesis and transcription.
Citation Phytochemistry 2008; 69:738-46


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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Galvan IJ~Smith ML, 2008 growth (colony size) Chimaphylin [0.05 mg/mL] YPD hap a 4,439 Quantitative

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