Bockhorn J~Kinzy TG, 2008

Pubmed ID 19004804
Title Genome-wide screen of Saccharomyces cerevisiae null allele strains identifies genes involved in selenomethionine resistance.
Authors Jessica Bockhorn, Bharvi Balar, Dongming He, Eden Seitomer, Paul R Copeland, Terri Goss Kinzy
Abstract Selenomethionine (SeMet) is a potentially toxic amino acid, and yet it is a valuable tool in the preparation of labeled proteins for multiwavelength anomalous dispersion or single-wavelength anomalous dispersion phasing in X-ray crystallography. The mechanism by which high levels of SeMet exhibits its toxic effects in eukaryotic cells is not fully understood. Attempts to use Saccharomyces cerevisiae for the preparation of fully substituted SeMet proteins for X-ray crystallography have been limited. A screen of the viable S. cerevisiae haploid null allele strain collection for resistance to SeMet was performed. Deletion of the CYS3 gene encoding cystathionine gamma-lyase resulted in the highest resistance to SeMet. In addition, deletion of SSN2 resulted in both increased resistance to SeMet as well as reduced levels of Cys3p. A methionine auxotrophic strain lacking CYS3 was able to grow in media with SeMet as the only source of Met, achieving essentially 100% occupancy in total proteins. The CYS3 deletion strain provides advantages for an easy and cost-effective method to prepare SeMet-substituted protein in yeast and perhaps other eukaryotic systems.
Citation Proc. Natl. Acad. Sci. U.S.A. 2008; 105:17682-7


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Papers Phenotype Conditions Collection Tested mutants Data Details
Bockhorn J~Kinzy TG, 2008 growth (culture turbidity) selenomethionine [0.2 mM] hap a ~4,786 Quantitative only for hits

Curation history

July 18, 2013 Data requested.
Sept. 16, 2013 Data requested.
May 13, 2014 Data abandoned.
Feb. 16, 2015 Tested strains abandoned.
Feb. 16, 2015 Data to load.
Jan. 7, 2017 Data loaded.