Anand VC~Payne GS, 2009

Pubmed ID 19116312
Title Genome-wide analysis of AP-3-dependent protein transport in yeast.
Authors Vikram C Anand, Lydia Daboussi, Todd C Lorenz, Gregory S Payne
Abstract The evolutionarily conserved adaptor protein-3 (AP-3) complex mediates cargo-selective transport to lysosomes and lysosome-related organelles. To identify proteins that function in AP-3-mediated transport, we performed a genome-wide screen in Saccharomyces cerevisiae for defects in the vacuolar maturation of alkaline phosphatase (ALP), a cargo of the AP-3 pathway. Forty-nine gene deletion strains were identified that accumulated precursor ALP, many with established defects in vacuolar protein transport. Maturation of a vacuolar membrane protein delivered via a separate, clathrin-dependent pathway, was affected in all strains except those with deletions of YCK3, encoding a vacuolar type I casein kinase; SVP26, encoding an endoplasmic reticulum (ER) export receptor for ALP; and AP-3 subunit genes. Subcellular fractionation and fluorescence microscopy revealed ALP transport defects in yck3Delta cells. Characterization of svp26Delta cells revealed a role for Svp26p in ER export of only a subset of type II membrane proteins. Finally, ALP maturation kinetics in vac8Delta and vac17Delta cells suggests that vacuole inheritance is important for rapid generation of proteolytically active vacuolar compartments in daughter cells. We propose that the cargo-selective nature of the AP-3 pathway in yeast is achieved by AP-3 and Yck3p functioning in concert with machinery shared by other vacuolar transport pathways.
Citation Mol. Biol. Cell 2009; 20:1592-604

Datasets

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Papers Phenotype Conditions Collection Tested mutants Data Details
Anand VC~Payne GS, 2009 transport to/from vacuoles (proteolytic maturation of ALP in the vacuole) standard [standard] hap a/hap alpha ~4,871 Quantitative only for hits

Curation history

Data

July 16, 2013 Data requested.
Sept. 16, 2013 Data requested.
May 13, 2014 Data abandoned.
Feb. 16, 2015 Data to load.
May 7, 2017 Data loaded.

Tested strains

Feb. 16, 2015 Tested strains abandoned.