Burston HE~Conibear E, 2009

Pubmed ID 19506040
Title Regulators of yeast endocytosis identified by systematic quantitative analysis.
Authors Helen E Burston, Lymarie Maldonado-Báez, Michael Davey, Benjamen Montpetit, Cayetana Schluter, Beverly Wendland, Elizabeth Conibear
Abstract Endocytosis of receptors at the plasma membrane is controlled by a complex mechanism that includes clathrin, adaptors, and actin regulators. Many of these proteins are conserved in yeast yet lack observable mutant phenotypes, which suggests that yeast endocytosis may be subject to different regulatory mechanisms. Here, we have systematically defined genes required for internalization using a quantitative genome-wide screen that monitors localization of the yeast vesicle-associated membrane protein (VAMP)/synaptobrevin homologue Snc1. Genetic interaction mapping was used to place these genes into functional modules containing known and novel endocytic regulators, and cargo selectivity was evaluated by an array-based comparative analysis. We demonstrate that clathrin and the yeast AP180 clathrin adaptor proteins have a cargo-specific role in Snc1 internalization. We additionally identify low dye binding 17 (LDB17) as a novel conserved component of the endocytic machinery. Ldb17 is recruited to cortical actin patches before actin polymerization and regulates normal coat dynamics and actin assembly. Our findings highlight the conserved machinery and reveal novel mechanisms that underlie endocytic internalization.
Citation J. Cell Biol. 2009; 185:1097-110


Download the list of datasets
Paper Phenotype Condition Reference Collection Tested mutants Data Details
Burston HE~Conibear E, 2009 clathrin-mediated endocytosis (Snc1) standard hap a ~2,345 Quantitative only for hits
Burston HE~Conibear E, 2009 clathrin-mediated endocytosis (Snc1) standard hap alpha ~2,345 Quantitative only for hits

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Feb. 4, 2014 To request.
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