Askree SH~McEachern MJ, 2004

Pubmed ID 15161972
Title A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length.
Authors Syed H Askree, Tal Yehuda, Sarit Smolikov, Raya Gurevich, Joshua Hawk, Carrie Coker, Anat Krauskopf, Martin Kupiec, Michael J McEachern
Abstract Telomeres are nucleoprotein structures present at the ends of eukaryotic chromosomes that play a central role in guarding the integrity of the genome by protecting chromosome ends from degradation and fusion. Length regulation is central to telomere function. To broaden our knowledge about the mechanisms that control telomere length, we have carried out a systematic examination of approximately 4,800 haploid deletion mutants of Saccharomyces cerevisiae for telomere-length alterations. By using this screen, we have identified >150 candidate genes not previously known to affect telomere length. In two-thirds of the identified mutants, short telomeres were observed; whereas in one-third, telomeres were lengthened. The genes identified are very diverse in their functions, but certain categories, including DNA and RNA metabolism, chromatin modification, and vacuolar traffic, are overrepresented. Our results greatly enlarge the number of known genes that affect telomere metabolism and will provide insights into how telomere function is linked to many other cellular processes.
Citation Proc. Natl. Acad. Sci. U.S.A. 2004; 101:8658-63
Data abstract Deletion collection was assayed for telomere length via Southern blot and radioactive telomeric probe.


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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Askree SH~McEachern MJ, 2004 telomere length standard YPD hap a 4,781 Quantitative only for hits

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