Wyche TP~Bugni TS, 2014

Pubmed ID 25197007
Title Forazoline A: marine-derived polyketide with antifungal in vivo efficacy.
Authors Thomas P Wyche, Jeff S Piotrowski, Yanpeng Hou, Doug Braun, Raamesh Deshpande, Sean McIlwain, Irene M Ong, Chad L Myers, Ilia A Guzei, William M Westler, David R Andes, Tim S Bugni
Abstract Forazoline A, a novel antifungal polyketide with in vivo efficacy against Candida albicans, was discovered using LCMS-based metabolomics to investigate marine-invertebrate-associated bacteria. Forazoline A had a highly unusual and unprecedented skeleton. Acquisition of (13)C-(13)C gCOSY and (13)C-(15)N HMQC NMR data provided the direct carbon-carbon and carbon-nitrogen connectivity, respectively. This approach represents the first example of determining direct (13)C-(15)N connectivity for a natural product. Using yeast chemical genomics, we propose that forazoline A operated through a new mechanism of action with a phenotypic outcome of disrupting membrane integrity.
Citation Angew. Chem. Int. Ed. Engl. 2014; 53:11583-6

Datasets

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Papers Phenotype Conditions Collection Tested mutants Data Details
Wyche TP~Bugni TS, 2014 growth (relative abundance in pooled culture) forazoline A [250 ug/ml] drug-sensitized hap a ~4,000 Quantitative

Curation history

Data

June 10, 2016 Data to request.
June 10, 2016 Data not relevant.

Tested strains

June 10, 2016 Tested strains to request.
June 10, 2016 Tested strains not relevant.