Sauerwald J~Rapaport D, 2015

Pubmed ID 26149385
Title Genome-Wide Screens in Saccharomyces cerevisiae Highlight a Role for Cardiolipin in Biogenesis of Mitochondrial Outer Membrane Multispan Proteins.
Authors Julia Sauerwald, Tobias Jores, Michal Eisenberg-Bord, Silvia Gabriela Chuartzman, Maya Schuldiner, Doron Rapaport
Abstract A special group of mitochondrial outer membrane (MOM) proteins spans the membrane several times via multiple helical segments. Such multispan proteins are synthesized on cytosolic ribosomes before their targeting to mitochondria and insertion into the MOM. Previous work recognized the import receptor Tom70 and the mitochondrial import (MIM) complex, both residents of the MOM, as required for optimal biogenesis of these proteins. However, their involvement is not sufficient to explain either the entire import pathway or its regulation. To identify additional factors that are involved in the biogenesis of MOM multispan proteins, we performed complementary high-throughput visual and growth screens in Saccharomyces cerevisiae. Cardiolipin (CL) synthase (Crd1) appeared as a candidate in both screens. Our results indeed demonstrate lower steady-state levels of the multispan proteins Ugo1, Scm4, and Om14 in mitochondria from crd1Δ cells. Importantly, MOM single-span proteins were not affected by this mutation. Furthermore, organelles lacking Crd1 had a lower in vitro capacity to import newly synthesized Ugo1 and Scm4 molecules. Crd1, which is located in the mitochondrial inner membrane, condenses phosphatidylglycerol together with CDP-diacylglycerol to obtain de novo synthesized CL molecules. Hence, our findings suggest that CL is an important component in the biogenesis of MOM multispan proteins.
Citation Mol. Cell. Biol. 2015; 35:3200-11


Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Sauerwald J~Rapaport D, 2015 transport to mitochondria (Om14-GFP) standard SD + Met hap a ~5,000 Discrete
Sauerwald J~Rapaport D, 2015 protein insertion into the MOM (Ura3-HA-Om14-SL17) standard SD + Met hap a ~5,000 Quantitative only for hits

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