Bianchi MM~Frontali L, 2001

Pubmed ID 11746602
Title Large-scale phenotypic analysis reveals identical contributions to cell functions of known and unknown yeast genes.
Authors M M Bianchi, S Ngo, M Vandenbol, G Sartori, A Morlupi, C Ricci, S Stefani, G B Morlino, F Hilger, G Carignani, P P Slonimski, L Frontali
Abstract Sequencing of the yeast genome has shown that about one-third of the yeast ORFs code for unknown proteins. Many other have similarity to known genes, but still the cellular functions of the gene products are unknown. The aim of the B1 Consortium of the EUROFAN project was to perform a qualitative phenotypic analysis on yeast strains deleted for functionally orphan genes. To this end we set up a simple approach to detect growth defects of a relatively large number of strains in the presence of osmolytes, ethanol, high temperature, inhibitory compounds or drugs affecting protein biosynthesis, phosphorylation level or nucleic acids biosynthesis. We have now developed this procedure to a semi-quantitative level, we have included new inhibitors, such as hygromycin B, benomyl, metals and additional drugs interfering with synthesis of nucleic acids, and we have performed phenotypic analysis on the deleted strains of 564 genes poorly characterized in respect to their cellular functions. About 30% of the deleted strains showed at least one phenotype: many of them were pleiotropic. For many gene deletions, the linkage between the deletion marker and the observed phenotype(s) was studied by tetrad analysis and their co-segregation was demonstrated. Co-segregation was found in about two-thirds of the analysed strains showing phenotype(s).
Citation Yeast 2001; 18:1397-412
Notes The list of hits includes only mutant strains that show a consistent phenotype in Mat-a and Mat-alpha backgrounds.

Datasets

Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Bianchi MM~Frontali L, 2001 growth (undefined) Thialsine [0.15 mg/ml] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) DL-C-allyl-glycine hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) azaserine [0.53 ug/ml] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) benomyl [40 ug/ml] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) calcium dichloride [0.7 M] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) caffeine [0.1% w/v] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) cadmium dichloride [55 uM] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) cobalt dichloride [0.6 mM] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) cordycepin [0.016 mg/ml] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) caesium chloride [100 mM] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) cycloheximide [0.18 ug/ml] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) daunorubicin [0.05 mg/ml] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) ethanol [8-10% v/v], glucose [1 / (10 h)] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) diazo-oxo-norleucine hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) ethanol [4-6% v/v] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) sodium fluoride [35 mM] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) flucytosine [0.02 mg/ml] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) 3-Fluoro-DL-tyrosine [0.025 mg/ml] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) 5-fluorouracil [0.02 mg/ml] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) mercury dichloride [0.25 mM] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) hygromycin B [50-80 ug/ml] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) potassium chloride [1.2-1.5 M] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) sodium chloride [1.0-1.2 M] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) quinolin-8-ol [26 ug/ml] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) patulin [0.22 mg/ml] hap a/hap alpha ~295 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) glucitol [1.8-2.0 M], sodium chloride [0.3 M] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) glucitol [1.8-2.0 M] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) temperature [52ºC] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) 3-(2-Thienyl)-dl-alanine [0.013 mg/ml] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) trifluoperazine [15 uM] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) temperature [52ºC], temperature [38ºC], time [30-40 min] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) vanadate(3-) [3-5 mM] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) verrucarin A [2.45 ug/ml] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) DL-p-fluorophenyl-alanine hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) 5-fluorotryptophan [0.1 mg/ml] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) sodium chloride [1.0-1.2 M], sodium chloride [0.3 M] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) sodium chloride [1.0-1.2 M], sodium chloride [0.3 M] hap a/hap alpha ~564 Discrete
Bianchi MM~Frontali L, 2001 growth (undefined) potassium chloride [1.2-1.5 M], sodium chloride [0.3 M] hap a/hap alpha ~564 Discrete

Curation history

Data

Feb. 3, 2014 Waiting for tested.
Feb. 4, 2014 Ready to load.
Feb. 5, 2014 Loaded.
April 29, 2016 Not relevant.

Tested strains

Feb. 3, 2014 Request sent.
Feb. 4, 2014 Ready to load.
Feb. 5, 2014 Loaded.
April 29, 2016 Not relevant.