Tucker CL~Fields S, 2004

Pubmed ID 18629161
Title Quantitative genome-wide analysis of yeast deletion strain sensitivities to oxidative and chemical stress.
Authors Chandra L Tucker, Stanley Fields
Abstract Understanding the actions of drugs and toxins in a cell is of critical importance to medicine, yet many of the molecular events involved in chemical resistance are relatively uncharacterized. In order to identify the cellular processes and pathways targeted by chemicals, we took advantage of the haploid Saccharomyces cerevisiae deletion strains (Winzeler et al., 1999). Although ~4800 of the strains are viable, the loss of a gene in a pathway affected by a drug can lead to a synthetic lethal effect in which the combination of a deletion and a normally sublethal dose of a chemical results in loss of viability. WE carried out genome-wide screens to determine quantitative sensitivities of the deletion set to four chemicals: hydrogen peroxide, menadione, ibuprofen and mefloquine. Hydrogen peroxide and menadione induce oxidative stress in the cell, whereas ibuprofen and mefloquine are toxic to yeast by unknown mechanisms. Here we report the sensitivities of 659 deletion strains that are sensitive to one or more of these four compounds, including 163 multichemicalsensitive strains, 394 strains specific to hydrogen peroxide and/or menadione, 47 specific to ibuprofen and 55 specific to mefloquine.We correlate these results with data from other large-scale studies to yield novel insights into cellular function.
Citation Comp. Funct. Genomics 2004; 5:216-24

Datasets

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Tucker CL~Fields S, 2004 growth (culture turbidity) hydrogen peroxide [2 mM] hap alpha ~4,800 None

Curation history

Data

Feb. 20, 2019 To request.

Tested strains

Feb. 20, 2019 To request.