Breslow DK~Weissman JS, 2008

Pubmed ID 18622397
Title A comprehensive strategy enabling high-resolution functional analysis of the yeast genome.
Authors David K Breslow, Dale M Cameron, Sean R Collins, Maya Schuldiner, Jacob Stewart-Ornstein, Heather W Newman, Sigurd Braun, Hiten D Madhani, Nevan J Krogan, Jonathan S Weissman
Abstract Functional genomic studies in Saccharomyces cerevisiae have contributed enormously to our understanding of cellular processes. Their full potential, however, has been hampered by the limited availability of reagents to systematically study essential genes and the inability to quantify the small effects of most gene deletions on growth. Here we describe the construction of a library of hypomorphic alleles of essential genes and a high-throughput growth competition assay to measure fitness with unprecedented sensitivity. These tools dramatically increase the breadth and precision with which quantitative genetic analysis can be performed in yeast. We illustrate the value of these approaches by using genetic interactions to reveal new relationships between chromatin-modifying factors and to create a functional map of the proteasome. Finally, by measuring the fitness of strains in the yeast deletion library, we addressed an enigma regarding the apparent prevalence of gene dispensability and found that most genes do contribute to growth.
Citation Nat. Methods 2008; 5:711-8


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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Breslow DK~Weissman JS, 2008 growth (pooled culture) standard SGA final selection medium hap a 4,179 Quantitative

Curation history


March 12, 2013 Loaded.

Tested strains

March 12, 2013 Loaded.